2.7.7.14: ethanolamine-phosphate cytidylyltransferase

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For detailed information about ethanolamine-phosphate cytidylyltransferase, go to the full flat file.

Reaction

Synonyms

CTP-phosphoethanolamine cytidylyltransferase, CTP:ethanolaminephosphate cytidylyltransferase, CTP:phosphoethanolamine CT, CTP:phosphoethanolamine cytidylyltransferase, CTP:phosphoethanolamine cytidylyltransferase gene, cytidylyltransferase, ethanolamine phosphate, ECT, ET, ethanolamine phosphate cytidylyltransferase, ethanolamine-phosphate cytidylyltransferase, Pcyt2, Pcyt2alpha, Pcyt2beta, PECT1, phosphoethanolamine cytidyltransferase, phosphoethanolamine cytidylyltransferase, phosphorylethanolamine transferase, Tb11.01.5730

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.7 Nucleotidyltransferases

                2.7.7.14 ethanolamine-phosphate cytidylyltransferase

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Results	in table
15	Activating Compound
5104	AA Sequence
1	Application
1	CAS Registry Number
12	Cloned(Commentary)
1	Crystallization (Commentary)
83	Disease/ Diagnostics
16	Expression
37	General Information
5	General Stability
30	Inhibitors
4	Ki Value [mM]
34	KM Value [mM]
21	Localization
10	Metals/Ions
14	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
349	Organism
7	Pathway
2	PDB ID
11	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
1	Posttranslational Modification
10	Protein Variants
13	Purification (Commentary)
3	Reaction
1	Reaction Type
43	Reference
92	Source Tissue
4	Specific Activity [micromol/min/mg]
2	Storage Stability
62	Substrates and Products (Substrate)
11	Subunits
69	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
11	Turnover Number [1/s]

General Stability

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General Stability on EC 2.7.7.14 - ethanolamine-phosphate cytidylyltransferase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

bovine serum albumin, 2% w/v, 1 mM CTP and 20 mM Mg2+, or 10% v/v glycerol stabilizes

Rattus norvegicus

-

642881

enzyme in crude postmicrosomal supernatant is quite stable towards freezing and thawing, but the highly purified enzyme loses 85-90% of its activity when frozen and thawed twice

Rattus norvegicus

-

642881

omission of DTT from buffers used in the later steps of purification results in severe loss of activity

Rattus norvegicus

-

642881

purified enzyme can be stabilized by the addition of 10% v/v glycerol or 2% bovine serum albumin

Rattus norvegicus

-

642886

quite stable towards freezing and thawing

Rattus norvegicus

-

642886