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2.7.7.12: UDP-glucose-hexose-1-phosphate uridylyltransferase

This is an abbreviated version!
For detailed information about UDP-glucose-hexose-1-phosphate uridylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.12

Reaction

UDP-alpha-D-glucose
+
alpha-D-galactose 1-phosphate
=
alpha-D-glucose 1-phosphate
 +
UDP-alpha-D-galactose

Synonyms

galactose 1-phosphate uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridyltransferase, galactose-1-phosphate uridylyltransferase, galactose-1-phosphate:uridyltransferase, GALT, GALT enzyme, GalT1, GalT2, hexose 1-phosphate uridyltransferase, hexose 1-phosphate uridylyltransferase, hexose-1-phosphate uridylyltransferase, UDP-Glc-hexose-1-P uridylyltransferase, UDP-glucose-hexose-1-P uridylyltransferase, UDPGlc:Gal-1-P uridylyltransferase, UDPglucose-hexose-1-phosphate uridylyltransferase, UDPglucose:alpha-D-galactose-1-phosphate uridylyltransferase, uridine diphosphoglucose:alpha-D-galactose-1-phosphate uridilyltransferase, uridyl transferase, uridyltransferase, uridylylgalactose-1-P uridylyltransferase, uridylyltransferase, hexose 1-phosphate

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.12 UDP-glucose-hexose-1-phosphate uridylyltransferase

Cloned

Cloned on EC 2.7.7.12 - UDP-glucose-hexose-1-phosphate uridylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 to 4fold overexpression in strain BL21(DE3)
-
cloning in Escherichia coli and coexpression in yeast of mutant heterodimers derived from galactosemic patients, use of different tags
-
construction and overexpression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues
-
expression as fusion protein with glutathione-S-transferase from Schistosoma japonicum in a bacterial expression system
-
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Sacchaormyces cerevisiae
expression of His- and 12CA5 epitope-tagged subunits of mutants R333W and Q188R in yeast, analysis of homo- and heterodimer formation of wild-type and mutant subunits
-
expression of mutant H166G in strain CA13, expression of mutant H166A in strain BL21(DE3)pLysS
-
expression of N314D mutants in human lymphoblasts
-
expression of wild-type and mutant H166G in strain BL21(DE3)
-
expression of wild-type and mutants as His-tagged proteins in Escherichia coli Bl21(DE3)
-
expression of wild-type and mutants in Saccharomyces cerevisiae
-
expression of wild-type and mutants in yeast
-
gene galt, DNA and amino acid sequence determination and analysis
-
gene GALT, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21(DE3) plysS
-
gene PAE1184, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) Codon Plus RIL
overexpressed in Escherichia coli
overexpression in strain BL21
-
overexpression of mutants Q168N and Q168R in strain Bl21(DE3)
-
overexpression of wild-type and mutant S135L as His-tagged proteins, 2fold lower expression than the wild-type, in a null-background yeast
-
overexpression of wild-type and mutants in strain BL21(DE3)pLysS
the GALT gene is located on chromosome 9p13
-
transient expression of reporter plasmids in HepG2 cells and NS20Y mouse neuroblastoma cells carrying varying fragments of the gene sequence, functional promotor search and analysis, DNA sequencing