2.7.6.3: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
This is an abbreviated version!
For detailed information about 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, go to the full flat file.
Word Map on EC 2.7.6.3
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2.7.6.3
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pyrophosphorylation
-
pyrophosphate
-
pterins
-
folic
-
dihydrofolate
-
bisubstrate
-
sulfa
-
dihydroneopterin
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ampcpp
-
tetrahydrofolate
-
induced-fit
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drug development
-
medicine
- 2.7.6.3
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pyrophosphorylation
- pyrophosphate
- pterins
-
folic
- dihydrofolate
-
bisubstrate
-
sulfa
- dihydroneopterin
- ampcpp
- tetrahydrofolate
-
induced-fit
- drug development
- medicine
Reaction
Synonyms
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase/dihydropteroate synthase, 6-hydroxy-7,8-dihydropterin pyrophosphokinase, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK), 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase, 6-hydroxymethylpterin pyrophosphokinase, 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase, 7,8-dihydropteroate-synthesizing enzyme, 7,8-dihydroxymethylpterin-pyrophosphokinase, ATP:2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine 6'-pyrophosphotransferase, cytHPPK/DHPS, dihydropterin pyrophosphokinase, FOLK, FtHPPK-DHPS, H2-pteridine-CH2-OH pyrophosphokinase, H2-pteridine-CH2OH pyrophosphokinase, HPPK, HPPK-DHPS, HPPK/DHPS, HPPK/dihydropteroate synthase, hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, hydroxymethyldihydropteridine pyrophosphokinase, hydroxymethyldihydropterin diphosphokinase/dihydropteroate synthase, hydroxymethyldihydropterin pyrophosphokinase (HPPK), hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase, mitHPPK/DHPS, More, PPPK, PPPK-DHPS, PVX_123230, pyrophosphokinase, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
ECTree
2.7.6.3 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.7.6.3 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-hydroxymethyl-7,8-dihydropterin + ATP
6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
6-hydroxymethyl-7,8-dihydropterin + MgATP2-
6-hydroxymethyl-7,8-dihydropterin diphosphate + MgAMP
biosynthesis of folate cofactors
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 7,8-dihydro-6-(diphosphooxymethyl)pteridine
-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + H+ + (7,8-dihydropterin-6-yl)methyl diphosphate
-
-
-
?
dATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
dAMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine
-
-
-
-
?
6-hydroxymethyl-7,8-dihydropterin + ATP
6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
tetrahydrofolate biosynthesis
-
-
?
6-hydroxymethyl-7,8-dihydropterin + ATP
6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
-
-
?
6-hydroxymethyl-7,8-dihydropterin + ATP
6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
folate biosynthesis
-
-
?
6-hydroxymethyl-7,8-dihydropterin + ATP
6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
biosynthesis of folic acid
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate
-
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate
-
-
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate
-
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate
-
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate
-
-
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine
-
-
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine
key step in biosynthesis of folic acid
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine
ATP binding is followed by binding of 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
-
-
?
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine
-
-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
enzyme binds ATP first, followed by 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
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?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
the product 6-hydroxymethyl-7,8-dihydropterin diphosphate is an intermediate in the pathway for folic acid biosynthesis
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?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
ordered bi-bi mechanism with ATP as the first substrate
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-
?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
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-
-
ir
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
-
-
ir
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
ordered bi bi mechanism where ATP binds first and 6-hydroxymethyl-7,8-dihydropterin diphosphate is released last
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-
?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
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-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropteridine
AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
-
-
-
ir
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, i.e. mitHPPK/DHPS, is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis, folate biosynthesis in Arabidopsis thaliana, overview
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-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
fourth step in folate biosynthesis, detailed overview
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-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
the enzyme is absolutely specific for the substrate
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
step in the folate biosynthetic pathway, overview
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
fourth step in folate biosynthesis, detailed overview
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
the enzyme is absolutely specific for the substrate
-
-
?
ATP + 6-hydroxymethyl-7,8-dihydropterin
AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
Q7CKD7
-
-
-
?
additional information
?
-
binding affinity for GTP and GMP, 75fold weaker than for ATP
-
-
?
additional information
?
-
-
binding affinity for GTP and GMP, 75fold weaker than for ATP
-
-
?
additional information
?
-
the enzyme does not hydrolyze alpha,beta-methyleneadenosine 5-triphosphate
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze alpha,beta-methyleneadenosine 5-triphosphate
-
-
?
additional information
?
-
the bifunctional enzyme comprises the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) activity and dihydropteroate synthase (DHPS) activity, EC 2.5.1.15, the latter is very low due, at least in part, to the lack of a key residue that interacts with the substrate p-aminobenzoic acid
-
-
?
additional information
?
-
the enzyme does not hydrolyze alpha,beta-methyleneadenosine 5-triphosphate
-
-
?
additional information
?
-
the bifunctional enzyme comprises the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) activity and dihydropteroate synthase (DHPS) activity, EC 2.5.1.15, the latter is very low due, at least in part, to the lack of a key residue that interacts with the substrate p-aminobenzoic acid
-
-
?
additional information
?
-
the bifunctional HPPK-DHPS enzyme catalyzes sequential steps in the folate biosynthesis, i.e. the 6-hydroxymethylpterin pyrophosphokinase reaction and the dihydropteroate synthase, DHPS, reaction, EC 2.5.1.15
-
-
?
additional information
?
-
coupled assay method development using reduction of DHPS reaction product dihydropteroate to tetrahydropteroate by excess dihydrofolate reductase with the cofactor NADPH, the oxidation of NADPH is monitored, overview
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-
?
additional information
?
-
Q7CKD7
HPPK is a key enzyme in the folate-biosynthetic pathway and is essential for microorganisms but absent from mammals
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-
?
additional information
?
-
-
HPPK is a key enzyme in the folate-biosynthetic pathway and is essential for microorganisms but absent from mammals
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-
?
