2.7.6.2: thiamine diphosphokinase

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For detailed information about thiamine diphosphokinase, go to the full flat file.

Reaction

Synonyms

At1g02880 (AtTPK1), At2g44750 (AtTPK2), ATP:thiamin pyrophosphotransferase, CA1462, hTPK1, Plasmodium falciparum thiamine pyrophosphokinase, pyrophosphokinase, pyrophosphokinase, thiamin, TDPK, thiamin kinase, thiamin pyrophosphokinase, thiamin pyrophosphotransferase, thiamin:ATP pyrophosphotransferase, thiamine diphosphokinase, thiamine kinase, thiamine pyrophokinase, thiamine pyrophosphokinase, thiaminokinase, TPK, Tpk1, TPTase, YcfN

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.6 Diphosphotransferases

                2.7.6.2 thiamine diphosphokinase

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Results	in table
1	Activating Compound
14443	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
5	Cofactor
5	Crystallization (Commentary)
87	Disease/ Diagnostics
1	Expression
3	General Information
1	General Stability
34	Inhibitors
3	Ki Value [mM]
38	KM Value [mM]
8	Localization
22	Metals/Ions
11	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
37	Organism
8	Pathway
14	PDB ID
10	pH Optimum
3	pH Range
2	pI Value
12	Protein Variants
13	Purification (Commentary)
3	Reaction
1	Reaction Type
35	Reference
50	Source Tissue
12	Specific Activity [micromol/min/mg]
50	Substrates and Products (Substrate)
8	Subunits
48	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
4	Temperature Range [°C]
2	Temperature Stability [°C]
18	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.6.2 - thiamine diphosphokinase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant enzyme in complex with ATP analogue AMP-PNP and thiamine, hanging drop vapor diffusion method, mixing of 500 nl of protein solution with 500 nl of reservoir solution containing 17.5-20% PEG 4000, 0.2 M MgCl2 , 0.1 M Tris, and 20% glycerol, pH 7.0-pH 7.5, and equilibration against 1 ml or reservoir solution, X-ray diffraction structure determination and analysis at 1.96-2.1 A resolution

Candida albicans

-

697004

complexed with thiamin diphosphate-Mg2+ or thiamin-Mg2+, hanging drop vapor diffusion method, using 1.15-1.3 M sodium citrate, 0.1 M Na-HEPES, pH 8.6, at 22°C

Mus musculus

-

722395

Crystallization experiments were set up by incubating mouse TPK with pyrithiamine and MgATP2- prior to adding the equilibrium mixture of enzyme, substrate, and products to the previously determined crystallization conditions.

Mus musculus

-

674760

recombinant enzyme, hanging drop vapor diffusion method

Mus musculus

-

642756

hanging-drop vapor diffusion method, crystals of the complex of native recombinant enzyme with thiamine

Saccharomyces cerevisiae

-

642754