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2.7.4.8: guanylate kinase

This is an abbreviated version!
For detailed information about guanylate kinase, go to the full flat file.

Word Map on EC 2.7.4.8

Reaction

ATP
+
GMP
=
ADP
 +
GDP

Synonyms

5'-GMP kinase, apo-EcGMPK, ATP: GMP phosphotransferase, ATP:GMP phosphotransferase, BmGK, CASK polypeptide, Cavbeta1b, Cavbeta2a, deoxyguanylate kinase, DLG1/SAP97, ecGMPK, GK, GK3, GKc, GKpm, GMK, GMK3, GMP kinase, GMPK, guanosine monophosphate kinase, guanosine monophosphate kinase (EcGMPK), guanylate kinase, guanylate kinase (GK), guanylate monophosphate kinase, GUK, kinase, guanylate (phosphorylating), MAGUK, MAGUK PSD-95, MAGUKs, membrane associated guanylate kinase, membrane associated guanylate kinase protein, membrane-associated guanylate kinase, membrane-associated guanylate kinases, MoGuk1, MoGuk2, More, organellar GK, post-synaptic density-95 membrane associated guanylate kinase, postsynaptic density protein-95 SH3 GK, PSD-95 GK, PSD-95 MAGUK, PSD-95alpha, PSD-95beta, Staphylococcus aureus guanylate monophosphate kinase, voltage-gated calcium channel beta1b, voltage-gated calcium channel beta2a, YGK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.8 guanylate kinase

Purification

Purification on EC 2.7.4.8 - guanylate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 isoenzymes purified by a method that includes GMP agarose chromatography and isoelectric focusing
-
method
method that includes DEAE-cellulose and Sephadex-75 chromatography
method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-100 chromatography
-
method that includes DEAE-cellulose, hydroxyapatite and Sephadex-75 chromatography
-
method that includes DEAE-cellulose, Sephadex-75 chromatography and isoelectric focusing
-
method that includes DEAE-Sephacel and Blue Sepharose CL 6B chromatography
-
method that includes DEAE-Sephacel, Cibacron-blue Sepharose and two Sephadex-75 chromatography
-
method that includes Sephadex and two DEAE-cellulose chromatography
-
Ni2+-NTA agarose affinity column chromatography
Ni2+-NTA agarose affinity column chromatography and gel filtration
-
partial
partial by a method that includes DEAE-cellulose chromatography
-
partial by a method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-200 chromatography
-
recombinant ecGMPK is purified by a two-step chromatography procedure involving affinity chromatography on Blue Sepharose and gel filtration
recombinant His-tagged enzyme from Escherichia coli BL21 (DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography, native enzyme from chloroplasts by gel filtration
recombinant His-tagged GK domain fragment of human DLG1 from Escherichia coli by anion exchange chromatography and gel filtration. The tag is cleaved by TEV protease
recombinant His6-tagged PSD-95 SH3-GK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant GST-tagged PSD-95 SH3-GK from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
-
recombinant N-terminally His6-tagged and SUMO-tagged enzyme from Escherichia coli strain BL21-(DE3)-pLysS by nickel affinity chromatography and gel filtration
recombinant protein
TALON metal affinity resin column chromatography
-