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2.7.4.6: nucleoside-diphosphate kinase

This is an abbreviated version!
For detailed information about nucleoside-diphosphate kinase, go to the full flat file.

Word Map on EC 2.7.4.6

Reaction

ATP
+
nucleoside diphosphate
=
ADP
 +
nucleoside triphosphate

Synonyms

AfNDK, Afu5g03490, ASNDK, At4g09320, At4g11010, At5g63310, HsNDK, kinase, nucleoside diphosphate (phosphorylating), mitochondrial nucleoside diphosphate kinase, MJ1265, More, NDK, NDK B, NDK-1, NDKB, NDP kinase, NDP kinase A, NDP kinase alpha, NDP kinase beta, NDPK, NDPK B, NDPK I, NDPK II, NDPK III, NDPK IIpn, NDPK In, NDPK-3, NDPK-B, NDPK-C, NDPK-D, NDPK-In, NDPK1, NDPK2, NDPK3, NDPK3a, NDPKA, NDPKB, NM23, NM23 metastasis suppressor, Nm23-H1, NM23-H2, NM23-H4, NME1, NME2, NME3, NME4, nucleoside 5'-diphosphate kinase, nucleoside diphosphate (UDP) kinase, nucleoside diphosphate kinase, nucleoside diphosphate kinase 1, nucleoside diphosphate kinase 2, nucleoside diphosphate kinase 3, nucleoside diphosphate kinase 3a, nucleoside diphosphate kinase A, nucleoside diphosphate kinase B, nucleoside diphosphate kinase C, nucleoside diphosphate kinase D, nucleoside diphosphate kinase-1, nucleoside diphosphate kinase-2, nucleoside diphosphate kinases alpha, nucleoside diphosphate kinases beta, nucleoside diphosphate phosphotransferase, nucleoside diphosphokinase, nucleoside-diphosphate kinase 3, nucleotide diphosphate kinase, nucleotide phosphate kinase, ORF454, Os12g36194, OsNDPK2, SwoH, TcNDPK1, TcNDPK3, UDP kinase, uridine diphosphate kinase, WSL12, YSS2 protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.6 nucleoside-diphosphate kinase

Purification

Purification on EC 2.7.4.6 - nucleoside-diphosphate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isozymes: NDP-kinase I and II
-
affinity chromatography on Blue Sepharose
-
affinity chromatography on Cibacron Red 3B-P-Sepharose
-
ammonium sulfate precipitation, Bio-Gel hydroxyapatite column chromatography, GTP-agarose column chromatography, and DEAE-Sephacelgel filtration
-
ATP-agarose column chromatography and Hi-Trap Q resin column chromatography
ATP-agarose column chromatography, and gel filtration
-
ATP-agarose-affinity column chromatography, and gel filtration
cytosolic and secreted enzymes 76fold and 238fold, respectively, recombinant His6-tagged enzyme by nickel affinity chromatography
-
expressed in E. coli
glutathione Sepharose column chromatography, and gel filtration
HisTrap column chromatography and Hitrap Q column chromatography
-
HiTrap chelating column chromatography and Superdex 75 gel filtration
-
HiTrap Ni2+ chelating column chromatography, gel filtration
liver, partial: thymus and heart
-
marked isoelectric variability with pIs from 5.4 to 8.3
-
muscle, partial
-
native enzyme from chloroplasts to homogeneity, from cytosol and membranes
native enzyme partially by mitochondrion preparation
native enzyme partially by mitochondrion preparation from protoplasts
-
Ni Sepharose 6 column chromatography and Superdex 200 gel filtration
Ni-chelating Sepharose column chromatography
-
Ni-NTA column chromatography
Ni2+-agarose resin column chromatography, gel filtration
Ni2+-NTA agarose affinity column chromatography
-
partial
partial, cytosolic enzyme
-
Q-Sepharose column chromatography
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli strain BL21Star(DE3) to homogeneity by ATP affinity chromatography and dialysis
-
recombinant enzyme from strain BL21(DE3) by anion exchange chromatography, ammonium sulfate fractionation, dialysis, and gel filtration
-
recombinant enzymes using His-tag
-
recombinant GST-tagged wild-type enzyme from Escherichia coli by glutathione affinity chromatography
-
recombinant HA-tagged chimeric mutants NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152 by one-step affinity chromatography using an ATP-resin
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
-
recombinant His-tagged NDPK from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography, cleavage of the His-tag by thrombin
recombinant His-tagged wild-type and mutant NDPKB by nickel affinity chromatography
-
recombinant His-tagged wild-type and selenomethionine-labeled enzymes to homogeneity from Escherichia coli by nickel affinity chromatography, cation exchange chromatography, and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant protein
recombinant protein using GST-tag
recombinant protein using His-tag
recombinant proteins
-
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography and gel filtration
-
recombinant wild-type and mutant enzymes by anion exchange chromatography
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3)
recombisnt proteins using GST-tag
several molecular species can be separated by electrophoresis or chromatofocusing
-
Talon metal affinity resin column chromatography and Sephacryl S300 gel filtration
-
Talon metal affinity resin column chromatography, and gel filtration
-