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2.7.4.6: nucleoside-diphosphate kinase

This is an abbreviated version!
For detailed information about nucleoside-diphosphate kinase, go to the full flat file.

Word Map on EC 2.7.4.6

Reaction

ATP
+
nucleoside diphosphate
=
ADP
 +
nucleoside triphosphate

Synonyms

AfNDK, Afu5g03490, ASNDK, At4g09320, At4g11010, At5g63310, HsNDK, kinase, nucleoside diphosphate (phosphorylating), mitochondrial nucleoside diphosphate kinase, MJ1265, More, NDK, NDK B, NDK-1, NDKB, NDP kinase, NDP kinase A, NDP kinase alpha, NDP kinase beta, NDPK, NDPK B, NDPK I, NDPK II, NDPK III, NDPK IIpn, NDPK In, NDPK-3, NDPK-B, NDPK-C, NDPK-D, NDPK-In, NDPK1, NDPK2, NDPK3, NDPK3a, NDPKA, NDPKB, NM23, NM23 metastasis suppressor, Nm23-H1, NM23-H2, NM23-H4, NME1, NME2, NME3, NME4, nucleoside 5'-diphosphate kinase, nucleoside diphosphate (UDP) kinase, nucleoside diphosphate kinase, nucleoside diphosphate kinase 1, nucleoside diphosphate kinase 2, nucleoside diphosphate kinase 3, nucleoside diphosphate kinase 3a, nucleoside diphosphate kinase A, nucleoside diphosphate kinase B, nucleoside diphosphate kinase C, nucleoside diphosphate kinase D, nucleoside diphosphate kinase-1, nucleoside diphosphate kinase-2, nucleoside diphosphate kinases alpha, nucleoside diphosphate kinases beta, nucleoside diphosphate phosphotransferase, nucleoside diphosphokinase, nucleoside-diphosphate kinase 3, nucleotide diphosphate kinase, nucleotide phosphate kinase, ORF454, Os12g36194, OsNDPK2, SwoH, TcNDPK1, TcNDPK3, UDP kinase, uridine diphosphate kinase, WSL12, YSS2 protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.6 nucleoside-diphosphate kinase

General Stability

General Stability on EC 2.7.4.6 - nucleoside-diphosphate kinase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
amino-terminal extension enhances dimer to hexamer assembly and stabilizes the structure in low salt
ATP stabilizes the enzyme at a concentration of 0.01 mM
benzamidine and PMSF stabilize during purification
-
bovine serum albumin enhances stability
-
guanidinium and many other salts stabilize the hexamer of mutant D93N with a half maximum effect of about 0.1 M, increasing protein thermostability
mutants with a basic amino acid at residue 114 (G114K, G114R) are resistant against heat inactivation as well as against low salt dissociation
NaCl stabilizes the A134E/A135E mutant enzyme, but not the wild-type enzyme
-
NaCl stabilizes the wild-type enzyme, but the E134A/E135A mutant
NDPK becomes sensitive to proteolysis only after its solubilization, NDPK solubilization from the outer membrane of liver mitochondria could be partially reversed by the addition of 3.3 mM MgCl2, complete rebinding of the enzyme after the addition of MgCl2 is observed when the mitochondrial washing and storage medium contains leupeptin
-
nucleoside diphosphate kinase prepared from Halobacterium salinarum is active and stable in the absence of salt. Recombinant nucleoside diphosphate kinase expressed in Escherichia coli requires salt for activation in vitro, but once it acquires the proper folding, it no longer requires the presence of salts for its activity and stability
-
the wild-type enzyme requires NaCl at higher than 2 M NaCl for refolding. Increasing the salt concentration gradually facilitates the recovery of enzyme activity, and the recovery at 3 M NaCl is maximal. The recovery at 4 M NaCl decreases slightly. No refolding of the HisN111L mutant is detected below 1 M NaCl and slow refolding is observed at 2 M NaCl, significant recovery occurs at higher than 3 M NaCl. The refolding efficiency at 4 M NaCl is as same as that of 3 M NaCl, differently from the wild-type enzyme
unstable in dilute solutions, bovine serum albumin stabilizes, not DTT
-