2.7.4.27: [pyruvate, phosphate dikinase]-phosphate phosphotransferase
This is an abbreviated version!
For detailed information about [pyruvate, phosphate dikinase]-phosphate phosphotransferase, go to the full flat file.
Reaction
Synonyms
AtRP1, bifunctional regulatory protein of pyruvate,orthophosphate dikinase, C4 chloroplast PPDK, C4ppdk, GRMZM2G004880, GRMZM2G131286, PDRP, PDRP1, PDRP2, PPDK regulatory protein, pyruvate orthophosphate dikinase regulatory protein, pyruvate, orthophosphate dikinase regulatory protein, pyruvate, orthophosphate dikinase regulatory protein 1, pyruvate,orthophosphate dikinase regulatory protein, pyruvate,Pi dikinase regulatory protein
ECTree
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Purification
Purification on EC 2.7.4.27 - [pyruvate, phosphate dikinase]-phosphate phosphotransferase
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ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration
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immobilized metal ion affinity chromatography (Ni2+)
partially purified, ammonium sulfate precipitation, dye ligand chromatography (Blue-Sepharose, agarose-blue dextran), gel filtration
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partially purified, ammonium sulfate precipitation, dye-ligand chromatography (Blue Sepharose), gel filtration, anion exchange chromatography
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recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) RIL by nickel affinity chromatography