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2.7.4.24: diphosphoinositol-pentakisphosphate 1-kinase

This is an abbreviated version!
For detailed information about diphosphoinositol-pentakisphosphate 1-kinase, go to the full flat file.

Word Map on EC 2.7.4.24

Reaction

ATP
+
1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate
=
ADP
+
1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate

Synonyms

ATP:5-diphospho-1D-myo-inositol-pentakisphosphate phosphotransferase, diphospho-myo-inositol pentakisphosphate 5-kinase, diphosphoinositol pentakisphosphate kinase, diphosphoinositol pentakisphosphate kinase 2, diphosphoinositol-pentakisphosphate 1/3-kinase, diphosphoinositol-pentakisphosphate kinase, EC 2.7.1.155, IP7 kinase, kinase (phosphorylating), diphosphoinositol 1,2,3,4,5-pentakisphosphate 5-, More, PP-InsP5 kinase, PP-IP5 kinase, PPIP5K, PPIP5K1, PPIP5K2, VIP, VIP1, VIP2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.24 diphosphoinositol-pentakisphosphate 1-kinase

Purification

Purification on EC 2.7.4.24 - diphosphoinositol-pentakisphosphate 1-kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
GSTrap HP column chromatography
native PPIP5K1 from rat brain by anion exchange and heparin affinity chromatography, followed by nickel affinity chromatography and sucrose gradient centrifugation
recombinant FLAG-tagged or untagged isozymes PPIP5K1 and PPIP5K2 from HEK-293 cells and isozyme PPIP5K1 from Escherichia coli, respectively
recombinant GST-tagged wild-type Saccharomyces cerevisiae Vip1 kinase domain and mutant Vip1 fused to human GSTIP6K1 from bacteria
recombinant wild-type and truncated mutant enzymes