2.7.4.21: inositol-hexakisphosphate 5-kinase

This is an abbreviated version!
For detailed information about inositol-hexakisphosphate 5-kinase, go to the full flat file.

Reaction

Synonyms

AtIPK2alpha, AtIpk2beta, ATP:1D-myo-inositol-hexakisphosphate phosphotransferase, AtVip1, AtVip2, diphosphoinositol pentakisphosphate kinase 2, diphosphoinositol pentakisphosphate synthetase, EC 2.7.1.152, EhIP6KA, IHPK2, inositol hexakisphosphate 5-kinase, inositol hexakisphosphate kinase, inositol hexakisphosphate kinase 1, inositol hexakisphosphate kinase 2, inositol hexakisphosphate kinase 3, inositol hexakisphosphate kinase type 2, inositol hexakisphosphate kinase-1, inositol hexakisphosphate kinase-2, inositol hexakisphosphate kinase-3, inositol polyphosphate kinase, inositol-hexakisphosphate kinase, InsP6 kinase, InsP6-kinase, InsP6K1, InsP6K2, InsP6K3, IP6 kinase, IP6 kinase 1, IP6K, IP6K1, IP6K2, IP6K3, Ipk2, IPMK, Kcs1, kinase (phosphorylating), inositol hexakisphosphate, More, Pi uptake stimulator, PiUS, PPIP5K1, PPIP5K2, RID-2

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.4 Phosphotransferases with a phosphate group as acceptor

                2.7.4.21 inositol-hexakisphosphate 5-kinase

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Results	in table
16	Activating Compound
20	AA Sequence
3	Application
1	CAS Registry Number
31	Cloned(Commentary)
10	Cofactor
3	Crystallization (Commentary)
179	Disease/ Diagnostics
3	Expression
97	General Information
15	IC50 Value
33	Inhibitors
2	kcat/KM [mM/s]
11	KM Value [mM]
31	Localization
10	Metals/Ions
8	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
102	Organism
1	Pathway
17	PDB ID
5	pH Optimum
1	Posttranslational Modification
36	Protein Variants
7	Purification (Commentary)
2	Reaction
4	Reaction Type
70	Reference
1	Renatured (Commentary)
104	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
63	Substrates and Products (Substrate)
13	Subunits
148	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.4.21 - inositol-hexakisphosphate 5-kinase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant MBP-tagged EhIP6KA residues 32-270, several crystal complexes of the IP6K including those that contain either large (Ins(1,3,4,5,6)P5 /InsP6) or small (Ins(1,4,5)P3) substrates, hanging drop vapor diffusion, 60 mg/ml protein in solution is mixed with reservoir solution containing 12% w/v PEG 3350, 50 mM NaH2PO4, pH 5.5 at 18°C, or by hanging drop vapor diffusion for one week against a well buffer containing 8% w/v PEG 3350, 100 mM Na3citrate, pH 5.2 at 25°C, followed by dilution microseeding for two weeks with a well buffer of 8% w/v PEG 3350, 100 mM Na3citrate, pH 5.2, and 8% ethylene glycol at 25°C, soaking of crystals in 22% w/v PEG 3350, 10 mM MgCl2, 10 mM ATP, 0.1 M sodium acetate, pH 5.2, and 20 mM InsP6, 10 mM Ins(1,3,4,5,6)P5 or 10 mM Ins(1,4,5)P3 for 3 days, X-ray diffraction structure determination and analysis, molecular replacement using MBP (1ez9) and IP3K (1w2c) as search models

Entamoeba histolytica

C4M387

739144

-

Homo sapiens

O43314

721882

N-terminally truncated enzyme mutant, residues 41-366, hanging drop vapor diffusion against a well buffer of 12% w/v PEG 3350, 20 mM MgCl2, 0.1 M HEPES, pH 7.0, 0,1 mM AMP-PNP, and 2 mM CdCl2 at 4°C, 3 days, 4°C, X-ray diffraction structure determination and analysis

Homo sapiens

O43314

738042