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2.7.4.21: inositol-hexakisphosphate 5-kinase

This is an abbreviated version!
For detailed information about inositol-hexakisphosphate 5-kinase, go to the full flat file.

Word Map on EC 2.7.4.21

Reaction

ATP
+
1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate
=
ADP
 +
1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate

Synonyms

AtIPK2alpha, AtIpk2beta, ATP:1D-myo-inositol-hexakisphosphate phosphotransferase, AtVip1, AtVip2, diphosphoinositol pentakisphosphate kinase 2, diphosphoinositol pentakisphosphate synthetase, EC 2.7.1.152, EhIP6KA, IHPK2, inositol hexakisphosphate 5-kinase, inositol hexakisphosphate kinase, inositol hexakisphosphate kinase 1, inositol hexakisphosphate kinase 2, inositol hexakisphosphate kinase 3, inositol hexakisphosphate kinase type 2, inositol hexakisphosphate kinase-1, inositol hexakisphosphate kinase-2, inositol hexakisphosphate kinase-3, inositol polyphosphate kinase, inositol-hexakisphosphate kinase, InsP6 kinase, InsP6-kinase, InsP6K1, InsP6K2, InsP6K3, IP6 kinase, IP6 kinase 1, IP6K, IP6K1, IP6K2, IP6K3, Ipk2, IPMK, Kcs1, kinase (phosphorylating), inositol hexakisphosphate, More, Pi uptake stimulator, PiUS, PPIP5K1, PPIP5K2, RID-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.21 inositol-hexakisphosphate 5-kinase

Cloned

Cloned on EC 2.7.4.21 - inositol-hexakisphosphate 5-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in NIH-OVCAR-3 cells
-
expression in Drosophila melanogaster
expression in Escherichia coli
expression in Escherichia coli and HEK-293T cells, additionally wild-type IHPK2 and the NLS mutant is cloned into the pCXN2 mammalian expression vector and electroporated into NIH-OVCAR-3 cells
-
expression in Escherichia coli, NIH-OVCAR-3 and Hey ovarian carcinoma cells, IP6K2 mRNA is not induced in NIH-OVCAR-3 cells following treatment with interferon-beta or gamma-irradiation, both gamma-irradiation and interferon-beta induce IP6K2 protein via a post-translational mechanism
-
expression in HeLa cells, PC12 cells, Jurkat T cells, OVCAR-3 cells, HL-60 cells and HEK-293 cells
-
expression of the wild-type enzyme in Saccharomyces cerevisiae mutant strains, overexpression of the GST-tagged or Myc-tagged enzyme in HEK-293, HeLa, and HCT116 cells, expression of wild-type and mutant enzymes in HEK-293 cells
-
gene Ihpk1, located on chromosome 9, has 6 exons, of which exon 1 is exclusively noncoding, the start codon is located in exon 2, and the stop codon is located in exon 6, DNA and amino acid sequence determination and analysis. Expressionin MEF cell lines
-
gene IP6K1
gene Ip6k1, expression of murine IP6K1 rescues yeast kcs1DELTA inositol auxotrophy. Nuclear recombinant expression of GFP-tagged wild type IP6K1 in IP6K1 knockout cells
gene ip6k1, quantitative real-time PCr enzyme expression analysis
gene IP6K1, recombinant coexpression of GST-tagged wild-type and mutant enzymes with Myc-tagged PLIN1 in HEK-293 cells, recombinant expression of Myc-tagged enzyme
gene IP6K1, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression of IP6K1 prevents enhancement of ubiquitylation by the expression of DDB1/Cul4A in HEK293 cells. IP6K1 expression of shRNA-resistant mouse IP6K1 in IP6K1 knockdown cells, overexpression of TAP-IP6K1 in HEK-293 cells
gene ip6k2, quantitative real-time PCr enzyme expression analysis
gene ip6k3, quantitative real-time PCr enzyme expression analysis
gene vip1, phylogenetic analysis, AtVip1 restores InsP7 synthesis in a Saccharomyces cerevisiae kcs1 mutant
-
gene vip2, phylogenetic analysis, AtVip2 restores InsP7 synthesis in a Saccharomyces cerevisiae kcs1 mutant
-
GST fusion protein is transfected into HEK293T cells
overexpression of wild-type and inactive mutant IHPK2 in ovarian carcinoma cell lines
-
recombinant co-overexpression of InsP6K2 and C-terminal residues 219-414 of TDP-43 from InsP6K2-pDsRed tag and TDP219-pEGFP tag plasmids in SHSY-5Y cells. Transfection of SHSY-5Y cells with a plasmid encoding a catalytically inactive, dominant-negative InsP6K2 (K2 K/A). HSP90 protein expression is reduced in the cytoplasmic fraction of TDP219- and InsP6K2-transfected cells. HSP90 expression is also significantly reduced in cells doubly transfected with TDP219M and InsP6K2 compared with control cells
recombinant expression of MBP-tagged EhIP6KA residues 32-270
recombinant expression of wild-type and mutant enzymes
recombinant expression of wild-type and mutant enzymes with a Flag-tag beta-adducin tail and MARCKS domain tagged to it, or tagged with actin-binding domain (1-275 aa), repeat domain (303-525 aa), and PH domain (2197-2307 aa) of beta2-spectrin. Tag cleavage by prescission protease