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2.7.4.16: thiamine-phosphate kinase

This is an abbreviated version!
For detailed information about thiamine-phosphate kinase, go to the full flat file.

Word Map on EC 2.7.4.16

Reaction

ATP
+
thiamine phosphate
=
ADP
+
thiamine diphosphate

Synonyms

ATP:thiamin-phosphate phosphotransferase, kinase, thiamin monophosphate (phosphorylating), More, thiamin monophosphatase, thiamin monophosphate kinase, thiamin monophosphokinase, thiamin phosphate kinase, thiamin pyrophosphokinase, thiamin-monophosphate kinase, thiamin-phosphate kinase, thiamine monophosphate kinase, thiamine monophosphokinase, ThiL, TP kinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.16 thiamine-phosphate kinase

Cloned

Cloned on EC 2.7.4.16 - thiamine-phosphate kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of the thiamine monophosphate kinase (thiL) overexpression plasmid, overexpression from pCLK710
-
encoded by thiL, thiL is not transcriptionally regulated by thiamine or thiamine diphosphate
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli Rosetta BL21(DE3)R3
gene THI80 or Pcal_0657, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha