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2.7.3.9: phosphoenolpyruvate-protein phosphotransferase

This is an abbreviated version!
For detailed information about phosphoenolpyruvate-protein phosphotransferase, go to the full flat file.

Word Map on EC 2.7.3.9

Reaction

phosphoenolpyruvate
+
protein histidine
=
pyruvate
 +
protein N-pros-phosphohistidine

Synonyms

EIC, EIN, enzyme I, enzyme I of the phosphotransferase system, More, non-sugar-specific phosphotransferase, PEP:sugar phosphotransferase system enzyme I, PEP:sugar PT, phosphoenolpyruvate protein phosphotransferase, phosphoenolpyruvate sugar phosphotransferase enzyme I, phosphoenolpyruvate-dependent phosphotransferase, phosphoenolpyruvate-dependent phosphotransferase system, phosphoenolpyruvate-dependent phosphotransferase system enzyme I, phosphoenolpyruvate-protein phosphotransferase, phosphoenolpyruvate:carbohydrate phosphotransferase system, phosphoenolpyruvate:glucose/mannose phosphotransferase system, phosphoenolpyruvate:glycose phosphotransferase system, phosphoenolpyruvate:glycose phosphotransferase system enzyme I, phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase, phosphoenolpyruvate:sugar phosphotransferase, phosphoenolpyruvate:sugar phosphotransferase system, phosphopyruvate-protein factor phosphotransferase, phosphopyruvate-protein phosphotransferase, phosphotransferase enzyme I, phosphotransferase system component enzyme I, phosphotransferase system enzyme I, phosphotransferase, phosphoenolpyruvate-protein, protein kinase enzyme I, PTS, ptsA, ptsI, sugar-PEP phosphotransferase enzyme I, YpqE

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.3 Phosphotransferases with a nitrogenous group as acceptor
                2.7.3.9 phosphoenolpyruvate-protein phosphotransferase

Cloned

Cloned on EC 2.7.3.9 - phosphoenolpyruvate-protein phosphotransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli C41 cells
-
expressed in Escherichia coli K-12 and Rosetta (DE3) cells
expressed in Escherichia coli strain JW2409-1
-
expressed in Rosetta (DE3) cells
-
expression in Escherichia coli
gene ptsI encoding the enzyme, DNA and amino acid sequence determination and analysis, transcriptional organization and expression analysis, the genes encoding PTS components, namely ptsI, ptsH, and ptsF belong to the fructose-PTS gene cluster, whereas ptsG and ptsS are located in two separate regions. A gene coding for the DeoR-type transcriptional regulator sugR is involved in the transcriptional regulation of the fructose-PTS cluster genes of the C. glutamicum genome, overview. Organization of the intergenic region between the genes ptsI and cg2118 containing the binding motifs A and B
gene ptsI encoding the enzyme, DNA and amino acid sequence determination and analysis, transcriptional organization and expression analysis, the genes encoding PTS components, namely ptsI, ptsH, and ptsF belong to the fructose-PTS gene cluster, whereas ptsG and ptsS are located in two separate regions. A gene coding for the DeoR-type transcriptional regulator sugR is involved in the transcriptional regulation of the fructose-PTS cluster genes of the Corynebacterium glutamicum genome, overview. Organization of the intergenic region between the genes ptsI and cg2118 containing the binding motifs A and B
gene ptsI, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, recombinant expression in Escherichia coli strain BL21(DE3)pLysS
in overproducing transformants
in overproducing transformants, also the N- and C- terminal domain of the enzyme were cloned and expressed separately
mutant enzyme H186D is expressed in Escherichia coli C41 cells
-
overexpression of His-tagged protein in Escherichia coli
-
the C-terminal and N-terminal domains of enzyme I are expressed in Escherichia coli BL21star(DE3) cells
-
the N-terminal domain of enzyme I from Streptomyces coelicolor is expressed in Escherichia coli BL21(DE3) cells