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2.7.3.2: creatine kinase

This is an abbreviated version!
For detailed information about creatine kinase, go to the full flat file.

Word Map on EC 2.7.3.2

Reaction

ATP
+
Creatine
=
ADP
 +
phosphocreatine

Synonyms

adenosine triphosphate-creatine transphosphorylase, adenosine-5'-triphosphate: creatine phosphotransferase, ATP-creatine transphosphorylase, ATP: creatine N-phosphotransferase, ATP:creatine phosphotransferase, B-type creatine kinase, BB-CK, BB-type creatine kinase, BCK, brain creatine kinase, brain type creatine kinase, brain-type CK, brain-type creatine kinase, CK, CK MM, CK-B, CK-BB, CK-MB, CK-MM, ckb, CKM, CKMB, CKMBI, CKMiMi, creatine kinase, creatine kinase B, creatine kinase M-type, creatine kinase MB, creatine kinase muscle type, creatine kinase-MB, creatine N-phosphotransferase, creatine phosphokinase, creatine phosphotransferase, creatinine kinase, creatinine kinase MB, hBBCK, hMMCK, kinase, creatine (phosphorylating), M-CK, M1-CK, MB-CK, MCK, Mi-CK, MiMi-CK, mit-CK, mitochondrial creatine kinase, MM-CK, MM-type creatine kinase, More, MtCK, muscle creatine kinase, muscle type creatine kinase, muscle-type creatine kinase, phosphocreatine kinase, plasma creatine kinase, PSCKM, recombinant human brain-type creatine kinase, rHBCK, RM-CK, s-type CK, sarcomeric CK, sMiCK, sMtCK, u-type CK, ubiquitous CK, ubiquitous MtCK, uMiCK, uMtCK, zMMCK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.3 Phosphotransferases with a nitrogenous group as acceptor
                2.7.3.2 creatine kinase

Temperature Stability

Temperature Stability on EC 2.7.3.2 - creatine kinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 60
the enzyme acctivity is stable from 0°C up to 45°C, inactivation at 65°C
23
-
isozyme MiMi-CK: 1 h, 15% loss of activity, 2 h, 34% loss of activity, 3 h, 66% loss of activity, isozyme BB-CK: 6 h, 32% loss of activity, isozyme MM-CK: no loss of activity
25 - 60
the wild type enzyme remains stable after 10 min at 25-45°C. The activity drops to about 70% and after 10 min at 50°C. The enzyme is almost completely inactive after 10 min at 60°C
25 - 65
the activity of the wild type enzyme has few changes after heat treatment for 10 min at temperatures below 48°C. A steep decrease of activity is observed between 48°C and 60°C
35
-
calf brain: 0.01 M 2-mercaptoethanol enhances stability in pH-range 6-8
36
-
mutant D54G, melting temperature
40 - 43
inactivation of recombinant mutant R151A
41
inactivation of recombinant mutant R147A
42.2
-
T0.5: 42.2°C wild-type hBBCK
51
-
inactivation above
51.9
melting temperature of mutant L110D
52.3
melting temperature of wild-type
52.9
melting temperature of mutant L121D
53
-
midpoint temperature of thermal inactivation of wild-type enzyme is 52.5°C
53 - 55
inactivation of recombinant wild-type enzyme
53.6
melting temperature of mutant L115D
56.9
-
muscle-type creatine kinase of Danio rerio is less stable compared to human muscle-type creatine kinase, T0.5: 56.9°C
57.2
-
T0.5: 57.2°C wild-type hMMCK
62
-
wild-type hMMCK gets completely inactivated above 62°C
additional information