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2.7.2.8: acetylglutamate kinase

This is an abbreviated version!
For detailed information about acetylglutamate kinase, go to the full flat file.

Word Map on EC 2.7.2.8

Reaction

ATP
+
N-acetyl-L-glutamate
=
ADP
+
N-acetyl-L-glutamyl 5-phosphate

Synonyms

acetylglutamate kinase, acetylglutamate phosphokinase, amino-acid acetyltransferase, argB, CcNAGK, EcNAGK, GRMZM2G132777, kinase, acetylglutamate (phosphorylating), mmNAGS/K, N-acetyl glutamate kinase, N-acetyl-glutamate 5-phosphotransferase, N-acetyl-L-glutamate 5-phosphotransferase, N-acetyl-L-glutamate kinase, N-acetyl-L-glutamate synthase/kinase, N-acetylglutamate 5-phosphotransferase, N-acetylglutamate kinase, N-acetylglutamate phosphokinase, N-acetylglutamate-5-phosphotransferase, N-acetylglutamic 5-phosphotransferase, NagK, NAGK1, NAGS-K, SYPA5-5 N-acetylglutamate kinase, xcNAGS/K, yNAGK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.8 acetylglutamate kinase

Purification

Purification on EC 2.7.2.8 - acetylglutamate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography and gel filtration by binding to the PII protein, Ni-nitrilotriacetic acid agarose to purify the recombinant protein
His-Select nickel affinity gel column chromatography
-
Ni Sepharose column chromatography
-
of the recombinant protein
recombinant enzyme
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli BL21(DE3) by nickel affinity chromatography
using Ni-NTA chromatography