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2.7.2.3: phosphoglycerate kinase

This is an abbreviated version!
For detailed information about phosphoglycerate kinase, go to the full flat file.

Word Map on EC 2.7.2.3

Reaction

ATP
+
3-phospho-D-glycerate
=
ADP
 +
3-phospho-D-glyceroyl phosphate

Synonyms

3-PGK, 3-phosphoglycerate 1-phosphotransferase, 3-phosphoglycerate kinase, 3-phosphoglycerate phosphokinase, 3-phosphoglyceric acid kinase, 3-phosphoglyceric acid phosphokinase, 3-phosphoglyceric kinase, AbPGK, ATP-3-phospho-D-glycerate-1-phosphotransferase, ATP:3-phospho-D-glycerate 1-phosphotransferase, ATP:D-3-phosphoglycerate 1-phosphotransferase, chl-PGK, glycerate 3-phosphate kinase, glycerophosphate kinase, HacPGK1, HacPGK2, HapPGK, hPGK, HsPGK, human 3-phosphoglycerate kinase, kinase (phosphorylating), phosphoglycerate, Mfer_0156, OsPGK2, P-glycerate kinase, PAS-PGK, PfPGK, PGK, PGK 1, PGK-1, PGK-2, pgk-B, PGK1, PGK2, Pgk3, Pgk5, PGKA, PGKase-1, PGKB, PGKC, pgkp1, pgkp2, phosphoglycerate kinase, phosphoglycerate kinase 1, phosphoglycerate kinase 2, phosphoglycerate kinase B, phosphoglycerate kinase-1, phosphoglyceric acid kinase, phosphoglyceric kinase, phosphoglycerokinase, PwPGK, SjPGK, SSO0527, testis-specific phosphoglycerate kinase 2, TRSC58_02767, X chromosome-linked phosphoglycerate kinase-1

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.3 phosphoglycerate kinase

Purification

Purification on EC 2.7.2.3 - phosphoglycerate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
101fold from skeletal muscle, 3780fold from erythrocytes
-
135fold
trout
-
14 days old leaves, 545fold purification of the chloroplastic and 530fold of the cytoplasmic isoenzyme
-
2 isozymes and the truncated glycosomal isozyme, recombinant from Escherichia coli, to near homogeneity
-
2 isozymes: PGKA and PGKB, from testis to homogeneity
-
250fold from liver, to homogeneity
-
36fold
Spirulina geitleri
-
375fold to homogeneity
-
660fold, to homogeneity
-
876fold, to homogeneity
-
99fold from muscle, 333fold from testis
-
ammonium sulfate precipitation, Ni-NTA column chromatography and Superdex S200 gel filtration
Pseudomonas sp. TACII 18
by anionic exchange column
-
by heat treatment, ion exchange chromatography, and gel filtration
by sonication
-
cytosolic, 160fold, and chloroplastic, 200fold, isozymes, to homogeneity
-
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
from brain
-
from liver and muscle
-
from muscle and liver
further purification of crystalline enzyme
-
further purification of the commercial product
-
heat treatment at 80°C is an effective first step in the purification of these recombinant enzyme from extracts of the Escherichia coli host
isoenzyme PGK1 and all 3 genetic variants of PGK2
-
isozymes PGK-1, PGK-2, to homogeneity
-
isozymes PGKA and PGKB
-
native from Zymomonas mobilis, and recombinant from Escherichia coli
-
native wild-type from Pseudomonas sp., and recombinant native and His-tagged enzyme from Escherichia coli as , to homogeneity
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
overview: purification procedure
-
partial
partial purification of the native enzyme
purification from diverse sources by affinity elution chromatography
-
Q Sepharose chromatography (natural enzyme) or ammonium sulfate precipitation, HiPrep Q column chromatography and Superdex 200 gel filtration (recombinant enzyme)
recombinant as glutathione-S-transferase-tagged enzyme from Escherichia coli, to homogeneity
recombinant as His-tagged enzyme from Escherichia coli
recombinant enzyme
-
recombinant enzyme from Escherichia coli
-
recombinant from Escherichia coli, to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatgraphy
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally His-tagged wild-type and truncated mutant enzymes and 80 amino acids long peptide corresponding to the PGKA N-terminal insertion to homogeneity from Escherichia coli strain BL21(DE3) by metal affinity chromatography
recombinant soluble His-tagged enzyme from Escherichia coli
recombinant wild-type from Escherichia coli JM 83, 97fold
recombinant, wild-type from Escherichia coli, 51.7fold
Strep-Tactin Sepharose column chromatography
to homogeneity
to homogeneity, by gel filtration
using ion-exchange chromatography
-
using Ni-NTA chromatography
variants of humans in New Guinea and a male in the Samoa Islands
-
wild-type and mutants purified by metal-affinity chromatography, mutants purified more than 98%
-