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2.7.2.11: glutamate 5-kinase

This is an abbreviated version!
For detailed information about glutamate 5-kinase, go to the full flat file.

Word Map on EC 2.7.2.11

Reaction

ATP
+
L-glutamate
=
ADP
+
L-glutamate 5-phosphate

Synonyms

ATP-L-glutamate 5-phosphotransferase, ATP:gamma-L-glutamate phosphotransferase, G5K, gamma-GK, gamma-glutamate kinase, gamma-glutamyl kinase, gamma-glutamylphosphate kinase, GK, GKA, glutamate kinase, glutamate-5-kinase, GPK, kinase (phosphorylating), glutamate, kinase, glutamate (phosphorylating), PRO1, proB, scGK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.11 glutamate 5-kinase

Purification

Purification on EC 2.7.2.11 - glutamate 5-kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
(NH4)2SO4 precipitation and a single ion-exchange chromatography step are used
-
by Ni2+ column and gel filtration
enzyme form GK 1
-
from strain BRL806, designated as gamma-glutamyl kinase w+ and from reductase-overproducing strain BRL1945, designated as gamma-glutamyl kinase DHPr
-
GST resin column chromatography
HisTrap column chromatography
Ni-Sepharose column chromatography
to homogeneity
using Ni-NTA chromatography