2.7.2.11: glutamate 5-kinase
This is an abbreviated version!
For detailed information about glutamate 5-kinase, go to the full flat file.
Word Map on EC 2.7.2.11
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2.7.2.11
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proba
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1-pyrroline-5-carboxylate
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l-proline
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osmotolerance
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delta1-pyrroline-5-carboxylate
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osmoprotection
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gpr
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catharanthus
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uprooted
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biotechnology
- 2.7.2.11
- proba
- 1-pyrroline-5-carboxylate
- l-proline
-
osmotolerance
- delta1-pyrroline-5-carboxylate
-
osmoprotection
- gpr
-
catharanthus
-
uprooted
- biotechnology
Reaction
Synonyms
ATP-L-glutamate 5-phosphotransferase, ATP:gamma-L-glutamate phosphotransferase, G5K, gamma-GK, gamma-glutamate kinase, gamma-glutamyl kinase, gamma-glutamylphosphate kinase, GK, GKA, glutamate kinase, glutamate-5-kinase, GPK, kinase (phosphorylating), glutamate, kinase, glutamate (phosphorylating), PRO1, proB, scGK
ECTree
2.7.2.11 glutamate 5-kinaseAdvanced search results
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results (Inhibitors
Inhibitors on EC 2.7.2.11 - glutamate 5-kinase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
5-oxoproline
AAK domain has a crater on the beta sheet C-edge that hosts the active centre and binds 5-oxoproline
iodoacetamide
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0.125 mM, 60% inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects
L-trans-pyrrolidine-2,4-dicarboxylic acid
84% residual activity at 1 mM
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NEM
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0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects
PCMB
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0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects
L-Pro
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enzyme from strain PAO1: 5 mM, 50% inhibition, complete inhibition at 30 mM, noncompetitive. Strain PAO 879, a proline-auxotroph mutant lacks a proline-inhibitable gamma-glutamyl kinase
L-Pro
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IC50: 0.08 mM, at room temperature. At low temperatures the inhibition switches over into allosteric activation and the biosynthesis of proline is started
L-proline
completely inhibits the activity of wild-type gamma-glutamyl kinase/gamma-glutamyl phosphate reductase at concentrations greater than 0.1mM, and inhibits it by 30% at 0.01mM
L-proline
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results indicate that the sensitivity of Chlamydomonas glutamte kinase to feedback inhibition by L-proline is much lower than that of similar microbial glutamte kinase
L-proline
GK activity is subject to feedback inhibition by proline
additional information
L-pipecolinic acid, D-pipecolinic acid, N-methyl-L-proline, cis-3-hydroxy-L-proline, trans-1-acetyl-4-hydroxy-L-proline, D-proline as well as L-citrulline and D,L-ornithine at 1 mM concentration do not inhibit the enzymatic conversion of L-glutamate
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additional information
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L-pipecolinic acid, D-pipecolinic acid, N-methyl-L-proline, cis-3-hydroxy-L-proline, trans-1-acetyl-4-hydroxy-L-proline, D-proline as well as L-citrulline and D,L-ornithine at 1 mM concentration do not inhibit the enzymatic conversion of L-glutamate
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additional information
no significant differences in growth between mutant yeast cells on SD agar medium with heat shock (50°C, 3 to 5 h), ethanol (16 to20%), and sorbitol (2.0 to 2.5 M) stresses, although the proline-accumulating cells are more resistant to these stresses than cells expressing wild-type GK
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additional information
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no significant differences in growth between mutant yeast cells on SD agar medium with heat shock (50°C, 3 to 5 h), ethanol (16 to20%), and sorbitol (2.0 to 2.5 M) stresses, although the proline-accumulating cells are more resistant to these stresses than cells expressing wild-type GK
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