2.7.2.11: glutamate 5-kinase
This is an abbreviated version!
For detailed information about glutamate 5-kinase, go to the full flat file.
Word Map on EC 2.7.2.11
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2.7.2.11
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proba
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1-pyrroline-5-carboxylate
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l-proline
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osmotolerance
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delta1-pyrroline-5-carboxylate
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osmoprotection
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gpr
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catharanthus
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uprooted
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biotechnology
- 2.7.2.11
- proba
- 1-pyrroline-5-carboxylate
- l-proline
-
osmotolerance
- delta1-pyrroline-5-carboxylate
-
osmoprotection
- gpr
-
catharanthus
-
uprooted
- biotechnology
Reaction
Synonyms
ATP-L-glutamate 5-phosphotransferase, ATP:gamma-L-glutamate phosphotransferase, G5K, gamma-GK, gamma-glutamate kinase, gamma-glutamyl kinase, gamma-glutamylphosphate kinase, GK, GKA, glutamate kinase, glutamate-5-kinase, GPK, kinase (phosphorylating), glutamate, kinase, glutamate (phosphorylating), PRO1, proB, scGK
ECTree
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General Information
General Information on EC 2.7.2.11 - glutamate 5-kinase
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malfunction
metabolism
physiological function
additional information
the DELTApro1 cells are more sensitive to various stresses than wild-type cells. Yeast cells with gene PRO1 deletion or expressing inactive enzyme display a defect for ribophagy but not for nonselective autophagy. Yeast strains deficient ribophagy are sensitive to nitrogen starvation
malfunction
Yeast cells expressing mutant enzymes D154N and I150T accumulate proline and show a higher tolerance to various stresses, including freezing, ethanol, osmotic pressure, and heat shock
gamma-glutamyl kinase is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway
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Chlamydomonas reinhardtii gamma-glutamte kinase is functional as demonstrated by successful complementation of Escherichia coli auxotroph JW0232 (strain in which the proB gene has been knocked-out)
physiological function
the enzyme has an alternative function independent of proline biosynthesis. Gene PRO1 genetically interacts with UBP3, which encodes ubiquitin-specific protease, and is required for selective autophagy of ribosomes (ribophagy). The enzyme activity is indispensable for ribophagy, ribophagy is important for cell survival during nitrogen starvation
physiological function
enzyme activation kills Mycobacterium tuberculosis via induction of proline and reactive oxygen species overproduction
physiological function
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enzyme activation kills Mycobacterium tuberculosis via induction of proline and reactive oxygen species overproduction
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conserved residue Asp156 plays an important role in the catalytic activity
additional information
residues Asp154 and Ile150 in the Saccharomyces cerevisiae gamma-glutamate kinase are important for allosteric regulation and the affinity with L-proline and L-glutamate, but not with ATP. The PUA domain itself is required for the full display of enzyme activity, but is not involved in the allosteric regulation and the affinity with substrates and proline. The proline-auxotrophic yeast strain BY4741DELTApro1DELTAcar2 that expresses the PUA domain deletion-mutant grows even on proline-free medium, although a growth defect is observed