2.7.2.11: glutamate 5-kinase
This is an abbreviated version!
For detailed information about glutamate 5-kinase, go to the full flat file.
Word Map on EC 2.7.2.11
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2.7.2.11
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proba
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1-pyrroline-5-carboxylate
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l-proline
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osmotolerance
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delta1-pyrroline-5-carboxylate
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osmoprotection
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gpr
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catharanthus
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uprooted
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biotechnology
- 2.7.2.11
- proba
- 1-pyrroline-5-carboxylate
- l-proline
-
osmotolerance
- delta1-pyrroline-5-carboxylate
-
osmoprotection
- gpr
-
catharanthus
-
uprooted
- biotechnology
Reaction
Synonyms
ATP-L-glutamate 5-phosphotransferase, ATP:gamma-L-glutamate phosphotransferase, G5K, gamma-GK, gamma-glutamate kinase, gamma-glutamyl kinase, gamma-glutamylphosphate kinase, GK, GKA, glutamate kinase, glutamate-5-kinase, GPK, kinase (phosphorylating), glutamate, kinase, glutamate (phosphorylating), PRO1, proB, scGK
ECTree
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Cloned
Cloned on EC 2.7.2.11 - glutamate 5-kinase
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an artificial bifunctional enzyme, gamma-glutamyl kinase/gamma-glutamyl phosphate reductase obtained by fusing the Escherichia coli genes proA and proB improves NaCl tolerance when expressed in Escherichia coli. The proB gene is fused to the 5'-end of the proA gene with a linker encoding five amino acids
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Escherichia coli DH5alpha strain proB cloning into pET-22b to yield pGKE, and overexpression
expressed in Escherichia coli as a His-tagged fusion protein
gene PRO1, cloning of mutants in Escherichia coli strain DH5alpha
proB cloning into pET-22b to yield pGKE and overexpression in Escherichia coli BL21(DE3)
proBA fusion gene and mutated proBA fusion gene expressed in Escherichia coli JM83