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2.7.2.11: glutamate 5-kinase

This is an abbreviated version!
For detailed information about glutamate 5-kinase, go to the full flat file.

Word Map on EC 2.7.2.11

Reaction

ATP
+
L-glutamate
=
ADP
+
L-glutamate 5-phosphate

Synonyms

ATP-L-glutamate 5-phosphotransferase, ATP:gamma-L-glutamate phosphotransferase, G5K, gamma-GK, gamma-glutamate kinase, gamma-glutamyl kinase, gamma-glutamylphosphate kinase, GK, GKA, glutamate kinase, glutamate-5-kinase, GPK, kinase (phosphorylating), glutamate, kinase, glutamate (phosphorylating), PRO1, proB, scGK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.11 glutamate 5-kinase

Cloned

Cloned on EC 2.7.2.11 - glutamate 5-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
an artificial bifunctional enzyme, gamma-glutamyl kinase/gamma-glutamyl phosphate reductase obtained by fusing the Escherichia coli genes proA and proB improves NaCl tolerance when expressed in Escherichia coli. The proB gene is fused to the 5'-end of the proA gene with a linker encoding five amino acids
-
cloned in pET22 and overexpressed in Escherichia coli
-
Escherichia coli DH5alpha strain proB cloning into pET-22b to yield pGKE, and overexpression
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 (lambdaDE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) pLysS cells
expression in Escherichia coli
-
expression of PRO1 in Escherichia coli M15(pREP4)
gene PRO1, cloning of mutants in Escherichia coli strain DH5alpha
proB cloning into pET-22b to yield pGKE and overexpression in Escherichia coli BL21(DE3)
proBA fusion gene and mutated proBA fusion gene expressed in Escherichia coli JM83