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2.7.1.95: kanamycin kinase

This is an abbreviated version!
For detailed information about kanamycin kinase, go to the full flat file.

Word Map on EC 2.7.1.95

Reaction

ATP
+
kanamycin
=
ADP
+
kanamycin 3'-phosphate

Synonyms

amino-glycoside-3-phosphotransferase II, aminoglycoside 3'-phosphotransferase, aminoglycoside phosphotransferase, aminoglycoside phosphotransferase 3'-IIIa, i.e. APH3'-IIIa, aminoglycoside phosphotransferase VIII, APH, aph(3'')-IIIa, APH(3')-IIIa, APH(3')II, AphA, kinase, kanamycin (phosphorylating), Neo, neomycin phosphotransferase, neomycin phosphotransferase II, neomycin phosphotransferase-II, neomycin-kanamycin phosphotransferase, NPT, NPT-II, NPTII

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.95 kanamycin kinase

Engineering

Engineering on EC 2.7.1.95 - kanamycin kinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R211A
site-directed mutagenesis
S146A
-
19% of the activity of phosphorylated wild-type, 90% of the activity of unphosphorylated wild-type, in presence of Ca2+
S146A/S160A
-
17% of the activity of phosphorylated wild-type, 85% of the activity of unphosphorylated wild-type, in presence of Ca2+
S146A/S160A/S215A
-
17% of the activity of phosphorylated wild-type, 60% of the activity of unphosphorylated wild-type, in presence of Ca2+
S215A
-
88% of the activity of phosphorylated wild-type, 67% of the activity of unphosphorylated wild-type, in presence of Ca2+
S95A/S160A
-
82% of the activity of phosphorylated wild-type, 95% of the activity of unphosphorylated wild-type, in presence of Ca2+
S146A
-
19% of the activity of phosphorylated wild-type, 90% of the activity of unphosphorylated wild-type, in presence of Ca2+
-
S146A/S160A
-
17% of the activity of phosphorylated wild-type, 85% of the activity of unphosphorylated wild-type, in presence of Ca2+
-
S146A/S160A/S215A
-
17% of the activity of phosphorylated wild-type, 60% of the activity of unphosphorylated wild-type, in presence of Ca2+
-
S215A
-
88% of the activity of phosphorylated wild-type, 67% of the activity of unphosphorylated wild-type, in presence of Ca2+
-
S95A/S160A
-
82% of the activity of phosphorylated wild-type, 95% of the activity of unphosphorylated wild-type, in presence of Ca2+
-