2.7.1.90: diphosphate-fructose-6-phosphate 1-phosphotransferase

This is an abbreviated version!
For detailed information about diphosphate-fructose-6-phosphate 1-phosphotransferase, go to the full flat file.

Reaction

Synonyms

6-phosphofructokinase (pyrophosphate), AtPFPalpha2, diphosphate-D-fructose-6-phosphate 1-phosphotransferase, FBPase, fructose 1,6-bisphosphatase, inorganic pyrophosphate-dependent phosphofructokinase, inorganic pyrophosphate-phosphofructokinase, PFKA, PFP, PfpA4, phosphotransferase, pyrophosphate-D-fructose 6-phosphate 1-, PPi-dependent 6-phosphofructokinase, PPi-dependent phosphofructokinase, PPi-PFK, pyrophosphate D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate-D-fructose 6-phosphate 1-phosphotransferase, pyrophosphate-D-fructose 6-phosphate phosphotransferase, pyrophosphate-dependent 6-phosphofructokinase, pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, pyrophosphate-dependent phosphofructo-1-kinase, pyrophosphate-dependent phosphofructokinase, pyrophosphate-fructose-6-phosphate-phosphotransferase, pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate: fructose 6-phosphate 1-phosphotransferase, pyrophosphate: fructose-6-phosphate 1-phosphotransferase, pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate:fructose 6-phosphate 1-phosphotransferase, pyrophosphate:fructose-6-phosphate 1-phosphotransferase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.90 diphosphate-fructose-6-phosphate 1-phosphotransferase

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Results	in table
73	Activating Compound
13967	AA Sequence
1	CAS Registry Number
17	Cloned(Commentary)
1	Cofactor
5	Expression
19	General Information
10	General Stability
194	Inhibitors
4	kcat/KM [mM/s]
52	Ki Value [mM]
172	KM Value [mM]
13	Localization
148	Metals/Ions
74	Molecular Weight [Da]
76	Natural Substrates/ Products (Substrates)
147	Organism
1	Oxidation Stability
10	Pathway
11	PDB ID
59	pH Optimum
5	pH Range
1	pH Stability
4	pI Value
14	Protein Variants
56	Purification (Commentary)
8	Reaction
1	Reaction Type
124	Reference
59	Source Tissue
41	Specific Activity [micromol/min/mg]
14	Storage Stability
245	Substrates and Products (Substrate)
37	Subunits
100	Synonyms
1	Systematic Name
40	Temperature Optimum [°C]
10	Temperature Stability [°C]
18	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.7.1.90 - diphosphate-fructose-6-phosphate 1-phosphotransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 50% v/v glycerol, less than 50% loss of activity within 2 months

Entamoeba histolytica

-

661689

-20°C, in 10 mM HEPES-NaOH buffer, pH 7.5, 10 mM 2-mercaptoethanol, 30% v/v glycerol, several months

Phaseolus vulgaris

-

642091

-20°C, in 10 mM Tris-acetate buffer, pH 7.3, 0.1 mM EDTA, 0.5 mM DTT, 17 mM KCl, plus 20% v/v glycerol, 6 months

Vigna radiata

-

642099

-20°C, in 50 mM HEPES buffer, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM EDTA, 1 mM MgCl2, 5 mM KCl, 30% ethylene glycol, more than 1 month

Triticum aestivum

-

642097

-20°C, in kappa-buffer with glycerol, 10% v/v, about 25% loss of activity within 6 months

Acholeplasma laidlawii

-

642080

-20°C, in salt-containing solution, 25% glycerol, t1/2: 1 year

Entamoeba histolytica

-

642100

-20°C, partially purified enzyme, loss of 25% activity after 1 month

Entamoeba histolytica

-

642074

-80°C, in 10 mM Tris-acetate buffer, pH 7.3, 0.1 mM EDTA, 0.5 mM DTT, 17 mM KCl, 6 months

Vigna radiata

-

642099

-80°C, purified enzyme, 25 mM Tris-HCl, pH 7.0, 20% glycerol v/v, 2 mM 2-mercaptoethanol, stable for at least 6 months

Ananas comosus

-

642109

-90°C, quick frozen in a dry-ice and methanol bath, at least 6 months

Glycine max

-

642090

0°C, in HEPES-glycol buffer, several days

Glycine max

-

642090

4°C, 10% loss of activity within 2 weeks

Oryza sativa

-

642095

4°C, in glycerol, 20% w/v, 20% loss of activity within 1 week

Ricinus communis

-

642089

4°C, without glycerol, 1 week, then slow decrease of activity over a period of weeks

Vigna radiata

-

642099