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2.7.1.90: diphosphate-fructose-6-phosphate 1-phosphotransferase

This is an abbreviated version!
For detailed information about diphosphate-fructose-6-phosphate 1-phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.90

Reaction

diphosphate
+
D-fructose 6-phosphate
=
phosphate
 +
D-fructose 1,6-bisphosphate

Synonyms

6-phosphofructokinase (pyrophosphate), AtPFPalpha2, diphosphate-D-fructose-6-phosphate 1-phosphotransferase, FBPase, fructose 1,6-bisphosphatase, inorganic pyrophosphate-dependent phosphofructokinase, inorganic pyrophosphate-phosphofructokinase, PFKA, PFP, PfpA4, phosphotransferase, pyrophosphate-D-fructose 6-phosphate 1-, PPi-dependent 6-phosphofructokinase, PPi-dependent phosphofructokinase, PPi-PFK, pyrophosphate D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate-D-fructose 6-phosphate 1-phosphotransferase, pyrophosphate-D-fructose 6-phosphate phosphotransferase, pyrophosphate-dependent 6-phosphofructokinase, pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, pyrophosphate-dependent phosphofructo-1-kinase, pyrophosphate-dependent phosphofructokinase, pyrophosphate-fructose-6-phosphate-phosphotransferase, pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate: fructose 6-phosphate 1-phosphotransferase, pyrophosphate: fructose-6-phosphate 1-phosphotransferase, pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase, pyrophosphate:fructose 6-phosphate 1-phosphotransferase, pyrophosphate:fructose-6-phosphate 1-phosphotransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.90 diphosphate-fructose-6-phosphate 1-phosphotransferase

Engineering

Engineering on EC 2.7.1.90 - diphosphate-fructose-6-phosphate 1-phosphotransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H415A
-
site-directed mutagenesis, reduced activity, increased Km for fructose 6-phosphate and decreased Km for diphosphate
H416A
-
site-directed mutagenesis, reduced activity, slightly increased Km for fructose 6-phosphate
K408A
-
site-directed mutagenesis, activity , decreased Km for diphosphate
M249A
-
site-directed mutagenesis, reduced activity and increased Km-value for fructose 6-phosphate compared to wild-type
M249I
-
site-directed mutagenesis, highly reduced activity, increased Km-value for fructose 6-phosphate compared to wild-type
M249L
-
site-directed mutagenesis, highly reduced activity and increased Km-value for fructose 6-phosphate compared to wild-type
R377A
-
site-directed mutagenesis, increased Km values for fructose 6-phosphate and diphosphate
R405A
-
site-directed mutagenesis, increased activity, kinetic properties similar to the wild-type
R423A
-
site-directed mutagenesis, decrease of kcat by 10000fold, 10fold increased Km for diphosphate, 126fold increased Km for fructose 6-phosphate
S392A
-
site-directed mutagenesis, activity and kinetic properties similar to the wild-type
Y420A
-
site-directed mutagenesis, reduced activity, increased Km values for fructose 6-phosphate and diphosphate
Y420F
-
site-directed mutagenesis, reduced activity, increased Km for fructose 6-phosphate, decreased Km for diphosphate
Y420H
-
site-directed mutagenesis, reduced activity, increased Km values for fructose 6-phosphate and diphosphate
additional information
-
construction of transgenic tobacco plants, functionally overexpressing the recombinant enzyme, show decrease in biomass formation, but no dramatic physiological or morphological alterations, and no significant reduction of seed production, endogenous plant enzyme activity EC 2.7.1.11 is not reduced, increased usage of storage starch