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2.7.1.78: polynucleotide 5'-hydroxyl-kinase

This is an abbreviated version!
For detailed information about polynucleotide 5'-hydroxyl-kinase, go to the full flat file.

Word Map on EC 2.7.1.78

Reaction

ATP
+
5'-dephospho-DNA
=
ADP
+
5'-phospho-DNA

Synonyms

5' end-specific RNA/DNA kinase, 5' polynucleotide kinase, 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, 5'-OH polynucleotide kinase, ATP:5'-dephosphopolynucleotide 5'-phosphatase, cleavage and polyadenylation factor I subunit, CLP1, Clp1-like protein, DNA 5'-hydroxyl kinase, DNA kinase, Grc3, HD-Pnk, HEAB, hPNKP, kinase (phosphorylating), polynucleotide 5'-hydroxyl, More, mPNKP, Nol9, PALF, PhoClp1, PNK, Pnk1, PNKP, polynucleotide 5'-hydroxyl kinase (phosphorylating), polynucleotide 5'-hydroxyl-kinase, polynucleotide kinase, polynucleotide kinase and aprataxin-like forkhead-associated, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA-specific polynucleotide kinase, Rnl1, RPNK, T4 PNK, T4 polynucleotide kinase, T4 polynucleotide kinase/phosphatase, tRNA endonuclease complex, TSEN

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.78 polynucleotide 5'-hydroxyl-kinase

Turnover Number

Turnover Number on EC 2.7.1.78 - polynucleotide 5'-hydroxyl-kinase

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008 - 1.6
3'-CMP
2.6
5'-dephospho-GAAAA-RNA
pH and temperature not specified in the publication
-
2.9
5'-dephospho-GC-RNA
pH and temperature not specified in the publication
-
0.072
5'-HO-CCGACCAACGAAGGT
wild type enzyme, at pH 7.0 and 37°C
0.077
5'-OH DNA
-
0.00083
5'-phospho-DNA
-
0.00005 - 2.9
ATP
198
GGGCC(AG)10GGCCC-fluorescein
Tequatrovirus T4
-
1.9 - 2.6
r(A20)
-
0.00283
[gamma-S]ATP
synthetic ribozyme
-
pH-independent, 30°C