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2.7.1.78: polynucleotide 5'-hydroxyl-kinase

This is an abbreviated version!
For detailed information about polynucleotide 5'-hydroxyl-kinase, go to the full flat file.

Word Map on EC 2.7.1.78

Reaction

ATP
+
5'-dephospho-DNA
=
ADP
 +
5'-phospho-DNA

Synonyms

5' end-specific RNA/DNA kinase, 5' polynucleotide kinase, 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, 5'-OH polynucleotide kinase, ATP:5'-dephosphopolynucleotide 5'-phosphatase, cleavage and polyadenylation factor I subunit, CLP1, Clp1-like protein, DNA 5'-hydroxyl kinase, DNA kinase, Grc3, HD-Pnk, HEAB, hPNKP, kinase (phosphorylating), polynucleotide 5'-hydroxyl, More, mPNKP, Nol9, PALF, PhoClp1, PNK, Pnk1, PNKP, polynucleotide 5'-hydroxyl kinase (phosphorylating), polynucleotide 5'-hydroxyl-kinase, polynucleotide kinase, polynucleotide kinase and aprataxin-like forkhead-associated, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA-specific polynucleotide kinase, Rnl1, RPNK, T4 PNK, T4 polynucleotide kinase, T4 polynucleotide kinase/phosphatase, tRNA endonuclease complex, TSEN

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.78 polynucleotide 5'-hydroxyl-kinase

Purification

Purification on EC 2.7.1.78 - polynucleotide 5'-hydroxyl-kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
10860fold, to near homogeneity
-
1500fold
-
2 mutant forms pseT 1 and pseT 47
Tequatrovirus T4
-
431fold
-
6717fold, to near homogeneity
-
His-Spin Protein Miniprep column chromatography, and gel filtration
Tequatrovirus T4
-
His60 Ni Superflow resin column chromatography
-
Ni-agarose chromatography
-
Ni-agarose column chromatography and Superdex 200 gel filtration
Ni-agarose column chromatography, and gel filtration
Ni-NTA agarose column chromatography
-
nickel-agarose chromatography
-
on a Ni-NTA-agarose column, the His10Smt3 tag is removed with the Smt3-specific protease Ulp1
over 1875fold, to homogeneity
-
partial, 35fold
-
partial, 960fold
-
polymin P precipitable enzyme, 1570fold
-
recombinant GST-tagged human Nol9 from Sf9 insect cells by glutathione affinity chromatography
-
recombinant His-tagged full length enzyme and recombinant truncated enzyme from Escherichia coli
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally His-tagged polynucleotide kinase and aprataxin-like forkhead-associated from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
the mutant proteins are purified using ProBond resin
to homogeneity
-
to near homogeneity
Tequatrovirus T4
-
using Strep-Tactin resin
-