2.7.1.78: polynucleotide 5'-hydroxyl-kinase

This is an abbreviated version!
For detailed information about polynucleotide 5'-hydroxyl-kinase, go to the full flat file.

Reaction

Synonyms

5' end-specific RNA/DNA kinase, 5' polynucleotide kinase, 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, 5'-OH polynucleotide kinase, ATP:5'-dephosphopolynucleotide 5'-phosphatase, cleavage and polyadenylation factor I subunit, CLP1, Clp1-like protein, DNA 5'-hydroxyl kinase, DNA kinase, Grc3, HD-Pnk, HEAB, hPNKP, kinase (phosphorylating), polynucleotide 5'-hydroxyl, More, mPNKP, Nol9, PALF, PhoClp1, PNK, Pnk1, PNKP, polynucleotide 5'-hydroxyl kinase (phosphorylating), polynucleotide 5'-hydroxyl-kinase, polynucleotide kinase, polynucleotide kinase and aprataxin-like forkhead-associated, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA-specific polynucleotide kinase, Rnl1, RPNK, T4 PNK, T4 polynucleotide kinase, T4 polynucleotide kinase/phosphatase, tRNA endonuclease complex, TSEN

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.78 polynucleotide 5'-hydroxyl-kinase

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Results	in table
32	Activating Compound
1445	AA Sequence
14	Application
1	CAS Registry Number
20	Cloned(Commentary)
104	Cofactor
8	Crystallization (Commentary)
162	Disease/ Diagnostics
30	General Information
10	General Stability
7	IC50 Value
148	Inhibitors
13	Ki Value [mM]
55	KM Value [mM]
9	Localization
114	Metals/Ions
29	Molecular Weight [Da]
84	Natural Substrates/ Products (Substrates)
103	Organism
51	PDB ID
44	pH Optimum
14	pH Range
1	pH Stability
3	pI Value
62	Protein Variants
37	Purification (Commentary)
9	Reaction
9	Reaction Type
99	Reference
39	Source Tissue
18	Specific Activity [micromol/min/mg]
8	Storage Stability
315	Substrates and Products (Substrate)
35	Subunits
130	Synonyms
1	Systematic Name
34	Temperature Optimum [°C]
2	Temperature Range [°C]
24	Temperature Stability [°C]
17	Turnover Number [1/s]

KM Value

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KM Value on EC 2.7.1.78 - polynucleotide 5'-hydroxyl-kinase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.099

5'-dephospho-GAAAA-RNA

Caenorhabditis elegans

P52874

pH and temperature not specified in the publication

739067

-

0.109

5'-dephospho-GC-RNA

Caenorhabditis elegans

P52874

pH and temperature not specified in the publication

739067

-

0.0028

5'-HO-CCGACCAACGAAGGT

Acetivibrio thermocellus

A3DJ38

wild type enzyme, at pH 7.0 and 37°C

737681

0.0025 - 0.0036

5'-hydroxyl poly(A)

2 entries

0.0034

5'-hydroxyl poly(C)

Homo sapiens

-

pH 8.4, 37°C

642021

0.016

5'-OH DNA

Pyrococcus horikoshii

Q57936

-

706727

0.0027 - 0.046

5'-OH-DNA

7 entries

0.021

5'-phospho-DNA

Pyrococcus horikoshii

Q57936

-

706727

0.2

ADP

2 entries

0.00069 - 3.4

ATP

21 entries

0.025

CTP

Escherichia phage T2

-

-

642004

0.00056

GGGCC(AG)10GGCCC-fluorescein

Tequatrovirus T4

P06855

-

695561

0.033

GTP

Escherichia phage T2

-

-

642004

0.5 - 2.5

Mg2+

2 entries

0.1

Mn2+

Escherichia phage T2

-

pH 6.0, 37°C

642006

0.008

oligo (dT)25

Bos taurus

-

polymin P precipitable enzyme, pH 7.5, 37°C

642025

-

0.0222 - 0.143

oligonucleotides

Tequatrovirus T4

-

depending on the 5'-base and the length of the oligonucleotide, pH 7.6, 37°C

642004

0.0013 - 0.0015

r(A20)

2 entries

-

0.015

UTP

Escherichia phage T2

-

-

642004

0.22 - 3.1

[gamma-S]ATP

2 entries

0.53

[gamma-S]GTP

Mus musculus

-

pH 8.4, 37°C

642017

additional information

additional information

2 entries

-

0.0025

5'-hydroxyl poly(A)

Homo sapiens

-

pH 8.4, 37°C

642021

0.0036

5'-hydroxyl poly(A)

Mus musculus

-

pH 8.4, 37°C

642017

0.0027

5'-OH-DNA

Tequatrovirus T4

-

wild-type, pH 7.6, 37°C

642018

0.0031

5'-OH-DNA

Tequatrovirus T4

-

mutant pseT 1, pH 7.6, 37°C

642018

0.0039

5'-OH-DNA

Rattus norvegicus

-

pH 5.5, 37°C

81073

0.0045

5'-OH-DNA

Tequatrovirus T4

-

mutant pseT 47, pH 7.6, 37°C

642018

0.0065

5'-OH-DNA

Tequatrovirus T4

-

pH 7.4, 37°C

642013

0.0076

5'-OH-DNA

Tequatrovirus T4

-

large DNA fragments released by nuclease treatment, pH 7.6, 37°C

642004

0.046

5'-OH-DNA

Rattus norvegicus

-

pH 5.5

642007

0.2

ADP

Tequatrovirus T4

-

dephosphorylation of single-stranded oligonucleotides, pH 7.6, 37°C

642004

0.2

ADP

Tequatrovirus T4

-

phosphate exchange reaction between nucleotides, reverse reaction, pH 7.6, 37°C

642004

0.00069

ATP

Rattus norvegicus

-

pH 5.5, 37°C

81073

0.002

ATP

Rattus norvegicus

-

pH 5.5, 37°C

642022

0.004

ATP

Bos taurus

-

-

642004

0.004

ATP

Bos taurus

-

pH 5.5, 37°C

642012, 642023

0.004

ATP

Tequatrovirus T4

-

phosphate exchange reaction between nucleotides, forward reaction, pH 7.6, 37°C

642004

0.007

ATP

Omegavirus omega

-

pH 6.5, 37°C

652544

0.013 - 0.14

ATP

Tequatrovirus T4

-

depending on DNA acceptor, pH 7.6, 37°C

642004

0.014

ATP

Escherichia phage T2

-

-

642004

0.016

ATP

Rattus norvegicus

-

pH 5.5

642007

0.02

ATP

Rhodothermus phage RM378

-

pH 6.0, 65°C

659503

0.04

ATP

Kostyavirus CJW1

-

pH 6.5, 37°C

652544

0.052

ATP

Bos taurus

-

polymin P precipitable enzyme, pH 7.5, 37°C

642025

0.065

ATP

Tequatrovirus T4

-

pH 7.4, 37°C

642013

0.104

ATP

Runella slithyformis

-

at pH 8.0 and 37°C

761386

0.152

ATP

Tequatrovirus T4

-

mutant pseT 1, pH 7.6, 37°C

642018

0.171

ATP

Tequatrovirus T4

-

mutant pseT 47, pH 7.6, 37°C

642018

0.188

ATP

Tequatrovirus T4

-

wild-type, pH 7.6, 37°C

642018

0.259

ATP

Caenorhabditis elegans

P52874

pH and temperature not specified in the publication

739067

0.5

ATP

Homo sapiens

-

pH 8.4, 37°C

642021

0.6

ATP

unidentified baculovirus

-

pH 5.0, 30°C

659328

3.4

ATP

synthetic ribozyme

-

pH-independent in the range pH 6.0-8.5, 30°C

642024

0.5

Mg2+

Escherichia phage T2

-

pH 6.0, 37°C

642006

2.5

Mg2+

Bos taurus

-

pH 5.5, 37°C

642012

0.0013

r(A20)

Rhodothermus phage RM378

-

pH 6.0, 65°C

659503

-

0.0015

r(A20)

Rhodothermus phage RM378

-

pH 6.0, 65°C

659503

-

0.22

[gamma-S]ATP

Mus musculus

-

pH 8.4, 37°C

642017

3.1

[gamma-S]ATP

synthetic ribozyme

-

pH-independent in the range pH 6.0-8.5, 30°C

642024

additional information

additional information

Tequatrovirus T4

-

-

642004

-

additional information

additional information

synthetic ribozyme

-

detailed reaction kinetics, pH- and temperature-in-/dependence

642024

-