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2.7.1.77: nucleoside phosphotransferase

This is an abbreviated version!
For detailed information about nucleoside phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.77

Reaction

A nucleotide
+
a 2'-deoxyribonucleoside
=
a nucleoside
 +
a 2'-deoxyribonucleoside 5'-phosphate

Synonyms

nonspecific nucleoside phosphotransferase, NPT, nucleotide:3'-deoxynucleoside 5'-phosphotransferase, phosphotransferase, nucleoside

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.77 nucleoside phosphotransferase

General Stability

General Stability on EC 2.7.1.77 - nucleoside phosphotransferase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
-
does not lose acttivity on lyophilization
-
no loss of activity after dialysis overnight against distilled water
-
not stable to SDS
-
stable in presence of 2 M urea, but not 4 M urea
-
storage in 20% acetone for 1 week without loss of activity
-
the enzyme can be isolated and handled only in the presence of detergents
-
the enzyme tolerates repeated freeze-thawing
-
tolerates repeated freezing and thawing
-
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
-