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2.7.1.67: 1-phosphatidylinositol 4-kinase

This is an abbreviated version!
For detailed information about 1-phosphatidylinositol 4-kinase, go to the full flat file.

Word Map on EC 2.7.1.67

Reaction

ATP
+
1-phosphatidyl-1D-myo-inositol
=
ADP
 +
1-phosphatidyl-1D-myo-inositol 4-phosphate

Synonyms

1-phosphatidyl-1D-myo-inositol 4-phosphotransferase, 56 kDa type II PtdIns 4-kinase, alpha type II PI 4-kinase, AtPI4KIIIalpha1, AtPI4KIIIbeta1, AtPI4KIIIbeta2, beta type II PI 4-kinase, beta-type phosphatidylinositol 4-kinase, kinase, phosphatidylinositol (phosphorylating), Lsb6, Lsb6p, phosphatidyl-inositol 4-kinase type IIIalpha, phosphatidylinositol 4 kinase, phosphatidylinositol 4 kinase IIIbeta, phosphatidylinositol 4-kinase, phosphatidylinositol 4-kinase beta, phosphatidylinositol 4-kinase beta1, phosphatidylinositol 4-kinase IIalpha, phosphatidylinositol 4-kinase IIbeta, phosphatidylinositol 4-kinase III alpha, phosphatidylinositol 4-kinase IIIalpha, phosphatidylinositol 4-kinase IIIbeta, phosphatidylinositol 4-kinase IIIbeta,, phosphatidylinositol 4-kinase type 2-alpha, phosphatidylinositol 4-kinase type II, phosphatidylinositol 4-kinase type II alpha, phosphatidylinositol 4-kinase type IIalpha, phosphatidylinositol 4-kinase type IIbeta, phosphatidylinositol 4-kinase type-III alpha, phosphatidylinositol kinase, phosphatidylinositol-4-kinase, phosphatidylinositol-4-kinase IIalpha, phosphatidylinositol-4-kinase III beta, phosphatidylinositol-4-kinase type II alpha, phosphatidylinositol-4-kinase type IIalpha, PI 4-kinase, PI 4-kinase type IIalpha, PI kinase, PI-4 kinase, PI4-kinase, PI4K, PI4K beta, PI4K IIalpha, PI4K IIbeta, PI4K IIIbeta, PI4K2, PI4K230, PI4K2A, PI4K55, PI4K92, PI4KA, PI4KB, PI4Kbeta, PI4Kbeta1, PI4Kgamma3, PI4Kgamma5, PI4KII, PI4KIIalpha, PI4KIIbeta, PI4KIII-beta, PI4KIIIalpha, PI4KIIIbeta, Pik1, Pik1p, PIK230, Pik2p, Pik4ca, PIK4IIalpha, PIK4IIbeta, PIK4IIIalpha, PIK4IIIbeta, PIK55, PIKCB, PtdIns 4-kinase, PtdIns 4-kinase beta, PtdIns4KIIalpha, Stt4p, type II phosphatidylinositol 4-kinase, type II phosphatidylinositol kinase, type II phosphoinositide 4-kinase, type II PI 4-kinase enzyme, type II PI4Kalpha, type II PtdIns 4-kinase, type IIalpha phosphatidylinositol 4-kinase, type III alpha phosphatidylinositol 4-kinase, type III phosphatidylinositol 4-kinase alpha, type III phosphatidylinositol 4-kinase beta, type III PI 4-kinase beta, type III PI4K, type III PI4Kbeta, type-II PI4K, type-III phosphatidylinositol 4-kinase beta1, type-III phosphatidylinositol 4-kinase beta2, type-III PI4K

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.67 1-phosphatidylinositol 4-kinase

Purification

Purification on EC 2.7.1.67 - 1-phosphatidylinositol 4-kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity, anion exchange column chromatography, and gel filtration
COS-7 cells transfected with the HA-tagged PI 4-kinase construct
enzyme type II
-
enzyme type IIalpha
-
FLAG M2 magnetic bead chromatography
-
glutathione S-transferase PI4Kbeta fusion protein
-
glutathione-Sepharose column chromatography
-
HIS-Select nickel affinity gel chromatography
mPIK-I and mPIK-III from membrane fraction and cPIK-I and cPIK-II from cytoslic fraction
-
Ni-NTA affinity chromatography
Ni-NTA agarose column chromatography
-
Ni-NTA agarose column chromatography and Mono-Q column chromatography
-
Ni-NTA His binding resin column chromatography
-
Ni-NTA resin column chromatography and Superdex S200 gel filtration
Ni-NTA resin column chromatography, and Superdex 200 gel filtration
Ni-NTA resin column chromatography, Mono Q column chromatography and Superdex S200 gel filtration
Ni2+-nitrilotriacetate resin affinity chromatography
-
nickel affinity column chromatography and Superdex S200 gel filtration
partial
partial, enzyme form QI and QII
-
phosphatidylinositol 4-kinase type I and type II
-
PI kinase I, II and III
-
recombinant His-tagged catalytic domain of isozyme PI4KIIIalpha from Sf9 cells by nickel affinity chromatography
-
type II enzyme
-
type III kinase, isoform PI4K92 is expressed as His6 tagged protein in Sf9 cells