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2.7.1.63: polyphosphate-glucose phosphotransferase

This is an abbreviated version!
For detailed information about polyphosphate-glucose phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.63

Reaction

(Phosphate)n
+
D-glucose
=
(Phosphate)n-1
+
D-glucose 6-phosphate

Synonyms

All1371, GMK, inorganic polyphosphate/ATP-glucomannokinase, inorganic polyphosphate:D-glucose 6-phosphotransferase, More, PGPTase, phosphotransferase, polyphosphate-glucose, poly P glucokinase, poly(P) glucokinase, poly(P)/ATP-glucomannokinase, PolyP-dependent glucokinase, polyP-GK, polyP-Glk, polyphosphate glucokinase, polyphosphate-D-(+)-glucose-6-phosphotransferase, polyphosphate-dependent glucokinase, polyphosphate-glucose 6-phosphotransferase, polyphosphate/ATP-glucomannokinase, PP-Glk, PPGK, SCO5059

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.63 polyphosphate-glucose phosphotransferase

Purification

Purification on EC 2.7.1.63 - polyphosphate-glucose phosphotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione Sepharose column chromatography, and gel filtration
not separable from ATP-glucokinase
-
partial
recombinant enzyme from Escherichia coli in several steps
-
recombinant enzyme fused with a family 3 cellulose-binding module 3.2fold from Escherichia coli strain BL21(DE3) in a one-step purification and immobilization on regenerated amorphous cellulose
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and His-tag cleavage with factor Xa
Talon metal affinity column chromatography and PD-10 gel filtration