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2.7.1.6: galactokinase

This is an abbreviated version!
For detailed information about galactokinase, go to the full flat file.

Word Map on EC 2.7.1.6

Reaction

ATP
+
alpha-D-galactose
=
ADP
 +
alpha-D-galactose 1-phosphate

Synonyms

atgalk, ATP:D-galactose-1-phosphotransferase, BiGalK, CLB.507001.110, CLB.510667.120, GAL1, Gal1p, galactokinase, galactokinase 1, galactose kinase, GALK, GALK1, GalKAmu, GalKSpe4, kinase (phosphorylating), galacto-, kinase, galacto- (phosphorylating), SCO3136

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.6 galactokinase

Temperature Stability

Temperature Stability on EC 2.7.1.6 - galactokinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 45
-
pH 7.3, 50 mM phosphate buffer, 10 mM 2-mercaptoethanol, 5% glycerol, 60 min, stable
30
-
10 min, no loss of activity
37
-
very little activity at 37°C
4 - 30
-
only 20% of maximal activity
40
-
pH 7.5, 10 min, stable up to
50.33
Tm-value, mutant enzyme M185L/Y379W
50.51
Tm-value, mutant enzyme M185L
51.3
Tm-value, mutant enzyme R228K
51.68
Tm-value, mutant enzyme R228M
52.07
Tm-value, mutant enzyme Y379W
52.31
52.5
Tm-value, mutant enzyme E174Q
52.7
Tm-value, mutant enzyme Q242S
53.83
Tm-value, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W
54
Tm-value, mutant enzyme E245S
54.41
Tm-value, mutant enzyme A334S
55
-
nearly inactive at 55°C
55.01
Tm-value, mutant enzyme G373S
55.1
Tm-value, mutant enzyme E244S
55.13
Tm-value, mutant enzyme E174D
55.17
Tm-value, mutant enzyme M180V
55.42
Tm-value, mutant enzyme R105M
55.72
Tm-value, wild-type enzyme
55.74
Tm-value, wild type enzyme
55.8
Tm-value, mutant enzyme L231S
55.81
Tm-value, mutant enzyme R366Q
55.93
Tm-value, mutant enzyme M60V
56.1
Tm-value, wild-type enzyme
57.14
Tm-value, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W
57.7
Tm-value, mutant enzyme M60V/M180V
58.08
Tm-value, mutant enzyme M180V/M60V/A334S
58.09
Tm-value, mutant enzyme M180V/M60V/A334S/G373S/D268E
58.11
Tm-value, mutant enzyme M180V/M60V/A334S/G373S
58.12
Tm-value, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S
65
-
over 76% of maximal activity
70
-
10 min, complete loss of activity without dithiothreitol
95
-
20 min, 2 mM dithiothreitol, 60% loss of activity