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2.7.1.56: 1-phosphofructokinase

This is an abbreviated version!
For detailed information about 1-phosphofructokinase, go to the full flat file.

Word Map on EC 2.7.1.56

Reaction

ATP
+
D-Fructose 1-phosphate
=
ADP
+
D-fructose 1,6-bisphosphate

Synonyms

1-PFK, 1PFK, D-fructose-1-phosphate kinase, FruC, fructose 1-phosphate kinase, fructose-1-phosphate kinase, kinase, 1-phosphofructo- (phosphorylating), PFK, PFK-1, PFK1, PFK2, pfkB, phosphofructokinase 1, phosphofructokinase-1

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.56 1-phosphofructokinase

Storage Stability

Storage Stability on EC 2.7.1.56 - 1-phosphofructokinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 3 days, 70% loss of activity
-
-20°C, 100 mM (NH4)2SO4, pH 7.5, at least 8 months
-
-20°C, 100 mM (NH4)2SO4, pH 7.5, several months, no loss of activity
-
-20°C, 50 mM phosphate buffer, pH 7.5, 1 year, no loss of activity
-
-20°C, crude enzyme extract prepared in water, at least 3 weeks
-
-80°C, 20% glycerol, 2 years, no loss of activity
-
4°C, 0.8-1.3 mg protein/ml in 50 mM phosphate buffer, pH 7, 0.5 mM dithiothreitol, 5-10% loss of activity
-
4°C, 1 week, about 30% loss of activity
-
4°C, a few days
-