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2.7.1.52: fucokinase

This is an abbreviated version!
For detailed information about fucokinase, go to the full flat file.

Word Map on EC 2.7.1.52

Reaction

ATP
+
L-fucose
=
ADP
+
beta-L-fucose 1-phosphate

Synonyms

ATP:6-deoxy-beta-L-galactose 1-phosphotransferase, ATP:6-deoxy-L-galactose 1-phospho transferase, Fkgp, FKP, fucokinase, fucokinase/L-fucose-1-P-guanylyltransferase, fucokinase:GDP-fucose pyrophosphorylase, fucose kinase, Fuk, kinase, fuco- (phosphorylating), L-fucokinase, L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase, L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase, L-fucokinase:GDP-fucose pyrophosphorylase, L-fucose kinase, L-fucose kinase/GDP-L-fucose pyrophosphorylase, More

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.52 fucokinase

Cloned

Cloned on EC 2.7.1.52 - fucokinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21star(DE3) cells
-
expression as fusion protein with maltose-binding protein in Escherichia coli strain BL21 (DE3)
-
expression in Escherichia coli with chaperones GroEL, GroES
-
expression of full-length protein and a deletion mutant lacking the first 363 amino acids of the N-terminus are expressed in Escherichia coli BL21 cells. Both proteins display fucokinase activity. Identification of amino acid sequence
functional expression of HA-tagged FKP in Saccharomyces cerevisiae strain W303-1A, the biosynthesis of GDP-D-Ara in the recombinant Saccharomyces cerevisiae strain harboring the FKP gene can occur through a mechanism akin to that of GDP-L-Fuc via the salvage pathway. Expression of a loss-of-function FKP mutant in yeast
-
two different transcripts of 3057 and 3270 base pairs expressed in COS-7 cells, only the longer splice variant is enzymatically active