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2.7.1.45: 2-dehydro-3-deoxygluconokinase

This is an abbreviated version!
For detailed information about 2-dehydro-3-deoxygluconokinase, go to the full flat file.

Word Map on EC 2.7.1.45

Reaction

ATP
+
2-dehydro-3-deoxy-D-gluconate
=
ADP
+
2-dehydro-3-deoxy-6-phospho-D-gluconate

Synonyms

2-dehydrogluconokinase, 2-keto-3-deoxy-D-gluconate kinase, 2-keto-3-deoxy-D-gluconic acid kinase, 2-keto-3-deoxygluconate kinase, 2-keto-3-deoxygluconokinase, 2-ketogluconate kinase, FlKin, HVO_0549, KDG kinase, KDGK, KDGK-1, ketodeoxygluconokinase, KGUK, KGUKCnec, kinase, 2-keto-3-deoxyglucono- (phosphorylating), More, Ta0122, TM0067

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.45 2-dehydro-3-deoxygluconokinase

Purification

Purification on EC 2.7.1.45 - 2-dehydro-3-deoxygluconokinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Butyl Toyopearl column chromatography and Superdex 200 column gel filtration
DEAE Sepharose column chromatography, Q Sepharose column chromatography, Affi-gel blue column chromatography, Sephadex G-75 gel filtration, and Mono Q HR 5/5 column chromatography
-
recombinant enzyme from Escherichia coli, by heat precipitation and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and desalting gel filtration
recombinant protein