2.7.1.45: 2-dehydro-3-deoxygluconokinase

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For detailed information about 2-dehydro-3-deoxygluconokinase, go to the full flat file.

Reaction

Synonyms

2-dehydrogluconokinase, 2-keto-3-deoxy-D-gluconate kinase, 2-keto-3-deoxy-D-gluconic acid kinase, 2-keto-3-deoxygluconate kinase, 2-keto-3-deoxygluconokinase, 2-ketogluconate kinase, FlKin, HVO_0549, KDG kinase, KDGK, KDGK-1, ketodeoxygluconokinase, KGUK, KGUKCnec, kinase, 2-keto-3-deoxyglucono- (phosphorylating), More, Ta0122, TM0067

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.45 2-dehydro-3-deoxygluconokinase

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Results	in table
3191	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
6	Cofactor
4	Crystallization (Commentary)
8	General Information
1	General Stability
1	Inhibitors
13	KM Value [mM]
22	Metals/Ions
13	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
105	Organism
15	Pathway
22	PDB ID
11	pH Optimum
2	pH Range
3	pH Stability
1	pI Value
1	Posttranslational Modification
3	Protein Variants
10	Purification (Commentary)
4	Reaction
1	Reaction Type
17	Reference
2	Source Tissue
8	Specific Activity [micromol/min/mg]
1	Storage Stability
37	Substrates and Products (Substrate)
12	Subunits
32	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
8	Temperature Stability [°C]
6	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.1.45 - 2-dehydro-3-deoxygluconokinase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

2.05 A resolution. Comparison with structure of Thermus thermophilus

Thermotoga maritima

Q9WXS2

695053

vapor diffusion method, crystal structure is determeined at 2.05 A resolution

Thermotoga maritima

Q9WXS2

749247

2 enzyme crystal forms, X-ray diffraction structure determination and analysis at 2.3 and 3.2 A resolution, respectively, molecular replacement, enzyme in complex with ATP, with 2-dehydro-3-deoxy-D-gluconate and ATP analogue adenosine 5'-(beta,gamma-imino)triphosphate, i.e. AMP-PNP, or with 2-dehydro-3-deoxy-D-gluconate and ATP, X-ray diffraction structure determination and analysis at 2.1 A resolution

Thermus thermophilus

-

659714

purified recombinant enzyme, free enzyme or in complex with ATP and 2-dehydro-3-deoxy-D-gluconate, method 1: automated microbatch method, 500 nl 23 mg/ml protein mixed with 500 nl screen solution containing 44% v/v methylpentanediol, 10% v/v dioxane, 0.1 M Na HEPES, pH 7.7, and covered with 0.015 ml silicone and parraffin oil mixture, 18°C, crystals grow to final dimensions within 1 month after appearance, method 2: sitting drop vapour diffusion method at 25°C, 0.001 ml 10 mg/ml protein solution mixed with equal volume of reservoir solution and equilibrated against 0.1 ml reservoir solution containing 28% v/v methylpentanediol, 10 mM CaCl2, 0.1 M trisodium citrate buffer, pH 5.6, or for cocrystallization of enzyme with ligands containing 0.35-0.45 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.1-3.2 A resolution

Thermus thermophilus

-

657469