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2.7.1.4: fructokinase

This is an abbreviated version!
For detailed information about fructokinase, go to the full flat file.

Word Map on EC 2.7.1.4

Reaction

ATP
+
D-fructose
=
ADP
 +
D-fructose 6-phosphate

Synonyms

At1g06020, At1g66430, At2g31390, At3g59480, At4g10260, At5g51830, AtFRK6, AtFRK7, CL6EHI_054510, cscK, D-fructokinase, D-fructose(D-mannose)kinase, FK, FK1, FK2, FRK, Frk1, Frk2, Frk3, Frk4, FRK5, FRK6, FRK7, fructokinase, Hore_18220, KHK, kinase, fructo- (phosphorylating), MdFRK1, MdFRK2, MEALZv4_0616, putative fructokinase, ROK FK, sacK1, Vc-FK, YdhR

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.4 fructokinase

Purification

Purification on EC 2.7.1.4 - fructokinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2-step purification leads to highly purified enzyme
-
3 forms: FK-I, FK-II and FK-III
-
400fold, FKI and FKII
-
FK1, FK2, FK3
-
homogeneity
-
isoenzyme FK-I, FK-II
-
isoenzyme fructokinase III
-
isoforms FRK1 and FRK2
-
partial
partial, 3 forms: fructokinase-Ia,-Ib,-II
-
purification of UTP-dependent enzyme: CTP, GTP and UTP are more effective as phosphate donors than ATP, organism also has a nonspecific fructokinase and a third fairly specific activity for ATP
-
purified using Ni-NTA affinity chromatography
recombinant protein
-
Sephadex G25 column chromatography
using metal chelate affinity chromatography
using Ni-NTA chromatography
using Ni-NTA-chromatography and gel filtration