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2.7.1.39: homoserine kinase

This is an abbreviated version!
For detailed information about homoserine kinase, go to the full flat file.

Word Map on EC 2.7.1.39

Reaction

ATP
+
L-homoserine
=
ADP
 +
O-phospho-L-homoserine

Synonyms

CglThrB, CThrB, DMR1, homoserine kinase, homoserine kinase (phosphorylating), HSK, kinase (phosphorylating), homoserine, kinase, homoserine (phosphorylating), Thr1, Thr1p, ThrB

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.39 homoserine kinase

Crystallization

Crystallization on EC 2.7.1.39 - homoserine kinase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme in apoform or with either methionine or ATPgammaS and cysteine, hanging drop vapor diffusion method, mixing of 0.002 ml of 16.2 mg/ml protein in 20 mM Tris, pH 7.5, 150 mM NaCl, 50 mM KCl, and 50 mM MgCl2 with 0.002 ml of well solution containing 0.25 M ammonium sulfate, 25% PEG 3350, and 0.1 M HEPES, pH 7.5, and equilibration against 0.1 ml of well solution, X-ray diffraction structure determination and analysis at 1.8-2.2 A resolution
crystal structure of the enzyme with ADP reveals a novel nucleotide binding fold. The N-terminal domain contains an unusual left-handed beta,alpha,beta unit, while the C-terminal domain has a central alpha-beta plait fold with an insertion of four helices
-
crystals are grown by the hanging drop vapor diffusion method
the enzyme ternary complexes with its amino acid substrate and ATP analogues determined by X-ray crystallography