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2.7.1.32: choline kinase

This is an abbreviated version!
For detailed information about choline kinase, go to the full flat file.

Word Map on EC 2.7.1.32

Reaction

ATP
+
choline
=
ADP
 +
phosphocholine

Synonyms

ATP/choline phosphotransferase, ATP:choline phosphotransferase, CEK1, CEK2, CEK3, chbA, CHETK-alpha, CHK, chk-alpha, Chka, CHKA2, ChKalpha, ChoK, ChoK alpha, ChoKalpha, CHOKalpha1, ChoKalpha2, ChoKbeta1, choline kinase, choline kinase alpa, choline kinase alpha, choline kinase alpha1, choline kinase alpha2, choline kinase beta, choline kinase beta1, choline kinase isoform alpha2, choline kinase-alpha, choline phosphokinase, choline-ethanolamine kinase, choline/ethanolamine kinase, choline/ethanolamine kinase 1, choline/ethanolamine kinase 2, choline/ethanolamine kinase 3, CK, CK-alpha, CK-alpha1, CK-alpha1/beta, CK-alpha2, CK-beta, CK1, CK2, CK3, CKA-2, CKalpha, CKalpha1, CKalpha2, ckb, CKbeta, CKbeta1, CKI1, CKI1-encoded choline kinase, CKII, ER-localized Cho kinase, EtnK, hChoK, kinase, choline (phosphorylating), LicA, LinfC/EK35, More, PfChoK, PfCK, protein LicA, putative choline kinase, sChoK, TbC/EK2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.32 choline kinase

Purification

Purification on EC 2.7.1.32 - choline kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cleared lysate is applied to a HisTrap column column pre-loaded with Ni2+. Unbound proteins are removed by washing the column with 50 mM Tris/HCl, pH 8.0, 300 mM NaCl and 10 mM imidazole, whereas TbC/EK1 and TbC/EK2 are eluted with an imidazole gradient in the same buffer. Fractions containing TbC/EK1 or TbC/EK2 are pooled, dialysed against 50 mM Tris/HCl and 300 mM NaCl and purified further through a Superdex 200 16/60 gel-filtration column.
-
Con A column chromatography, Affi-Gel Blue gel filtration and Mono Q column chromatography
-
copurification of choline kinase and ethanolamine kinase
-
glutathione affinity column chromatography, Ni-NTA column chromatography, and Superdex 200 gel filtration
gluthatione-Sepharose column chromatography, Resource Q column chromatography and Superdex-200 gel filtration
HisTrap column chromatography and Superdex 200 gel filtration
isoforms CKA-2, CKB-2
-
isoforms: CK I, CK II
-
multiple forms
-
Ni-nitrilotriacetic acid agarose affinity and gel filtration
-
Ni-NTA agarose resin column chromatography and 300SW gel filtration
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA resin column chromatography
overexpressed PfCK protein is purified to homogeneity using Ni-nitrilotriacetic acid agarose affinity and gel filtration chromatography
-
overview
partial
recombinant enzyme sChoK from Escherichia coli strain BL21(DE3)
recombinant enzyme, from Sf-9 insect cells
-
recombinant enzymes using His-tag
-
recombinant His-SUMO-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3)pLysS by nickel affinity chromatography, and dialysis, if necessary the tag is cleaved, followd by another nickel affinity chromatography step, and dialysis, the last step for all is gel filtration
recombinant His-SUMO-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3)pLysS by nickel affinity chromatography, and dialysis, if necessary the tag is cleaved, followed by another nickel affinity chromatography step, and dialysis, the last step for all is gel filtration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography and gel filtration
recombinant proteins using GST-tag
-
recombinant tagged choline kinase alpha1 residues 75-457 by affinity and ion exchange chromatography, and gel filtration
two isoforms with greatly different pI
-