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2.7.1.30: glycerol kinase

This is an abbreviated version!
For detailed information about glycerol kinase, go to the full flat file.

Word Map on EC 2.7.1.30

Reaction

ATP
+
glycerol
=
ADP
+
sn-glycerol 3-phosphate

Synonyms

AFUB_068560, ASTP , ATP-stimulated glucocorticoid-receptor translocation promoter , ATP: glycerol 3-phosphotransferase, ATP: glycerol-3-phosphotransferase, ATP:glycerol 3-phosphotransferase, ATP:glycerol 3-phosphotransferase , ATP:glycerol-3-phosphotransferase, GK, GK1, GK2, GK3, GK4, GK5, glcA, GLPK, glyceric kinase, glycerokinase, glycerol kinase, glycerol kinase 2, glycerol kinase 5, GLYK, GUT1, GYK, kinase, glycerol (phosphorylating), KpGlpK, More, slr1672, TbgGK, Tk-GK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.30 glycerol kinase

Storage Stability

Storage Stability on EC 2.7.1.30 - glycerol kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, stable for more than 1 year
-
-20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months
0°C, suspension of crystals, 10 mM glycerol, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.1 M potassium phosphate, pH 7.0, saturated with ammonium sulfate, stable for several years
-
4°C, crystallized suspension in 2.2 M ammonium sulfate, stable for several months
Candida mycoderma
-
4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 48 h, more than 50% loss of activity
-
4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 5 mM ADP, 10 mM MgSO4 and 0.1% (v/v) glycerol, 4 weeks, no loss of activity
-
as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years