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2.7.1.30: glycerol kinase

This is an abbreviated version!
For detailed information about glycerol kinase, go to the full flat file.

Word Map on EC 2.7.1.30

Reaction

ATP
+
glycerol
=
ADP
 +
sn-glycerol 3-phosphate

Synonyms

AFUB_068560, ASTP , ATP-stimulated glucocorticoid-receptor translocation promoter , ATP: glycerol 3-phosphotransferase, ATP: glycerol-3-phosphotransferase, ATP:glycerol 3-phosphotransferase, ATP:glycerol 3-phosphotransferase , ATP:glycerol-3-phosphotransferase, GK, GK1, GK2, GK3, GK4, GK5, glcA, GLPK, glyceric kinase, glycerokinase, glycerol kinase, glycerol kinase 2, glycerol kinase 5, GLYK, GUT1, GYK, kinase, glycerol (phosphorylating), KpGlpK, More, slr1672, TbgGK, Tk-GK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.30 glycerol kinase

Purification

Purification on EC 2.7.1.30 - glycerol kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
application of triazine dye affinity chromatography to large-scale purification
-
GSTrap FF column chromatography, gel filtration
-
HiTrapQ HP column, Bio-Scale CHT20-I column, all purification procedures are carried out at 277 K.
-
metal-chelate affinity chromatography using a Ni-NTA column, anion exchange chromatography using a Mono Q 5/50 GL column and gel filtration chromatography using s Superdex 200 10/30 GL column,all purification steps are performed in standard buffer (20 mM TrisHCl (pH 7.5), 10 mM glycerol, 1 mM beta-mercaptoethanol) at 4°C excluding the affinity chromatography purification
mutant enzyme G230D
native and mutant enzyme
-
Ni-NTA affinity resin column chromatography and gel filtration
-
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
nickel affinity column chromatography
normal and genetically altered enzyme
-
partial
recombinant enzyme
-
recombinant GST-tagged Gyk from Escherichia coli by glutathione affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain JM109 by nickel affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 90°C for 30 min, followed by ammonium sulfate fractionation, dialysis, and nickel affinity chromatography, then hydroxy apatite chromatography, gel ifltration, and again dialysis