2.7.1.26: riboflavin kinase

This is an abbreviated version!
For detailed information about riboflavin kinase, go to the full flat file.

Word Map on EC 2.7.1.26

2.7.1.26

mononucleotide

ammoniagenes

fadss

isoalloxazine

kyphoplasty

flavocoenzyme

flavinogenic

ribityl

davawensis

roseoflavin

famata

ctp-dependent

synthesis

drug development

Reaction

Synonyms

AtFMN/FHy, ATP: riboflavin kinase, ATP:riboflavin kinase, bifunctional riboflavin kinase/FMN adenylyltransferase, CaFADS, FAD synthetase, FADS, FK, flavokinase, flavokinase/FAD synthetase, flavokinase/flavin adenine dinucleotide synthetase, FMN adenylyltransferase, FMNAT, HsRFK, kinase, riboflavin, More, RFK, RibC, ribF, riboflavin kinase, riboflavin kinase/FMN adenylyltransferase, riboflavine kinase, RibR

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.26 riboflavin kinase

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Results	in table
4	Activating Compound
43101	AA Sequence
3	Application
1	CAS Registry Number
15	Cloned(Commentary)
4	Cofactor
6	Crystallization (Commentary)
34	Disease/ Diagnostics
16	General Information
4	General Stability
59	Inhibitors
45	kcat/KM [mM/s]
44	Ki Value [mM]
75	KM Value [mM]
2	Localization
23	Metals/Ions
12	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
40	Organism
10	Pathway
33	PDB ID
10	pH Optimum
2	pH Range
35	Protein Variants
16	Purification (Commentary)
3	Reaction
1	Reaction Type
40	Reference
14	Source Tissue
9	Specific Activity [micromol/min/mg]
2	Storage Stability
111	Substrates and Products (Substrate)
14	Subunits
42	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
3	Temperature Range [°C]
2	Temperature Stability [°C]
52	Turnover Number [1/s]

Purification

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Purification on EC 2.7.1.26 - riboflavin kinase

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3 entries

affinity chromatography

Vigna radiata var. radiata

641245

ammonium sulfate precipitation, phenyl Sepharose column chromatography, and DEAE-cellulose column chromatography

Corynebacterium ammoniagenes

partial

partial, affinity chromatography

Megasphaera elsdenii

641242

presence of ATP:riboflavin 5'-phosphotransferase and ATP:FMN adenylyltransferase on a single polypeptide

Corynebacterium ammoniagenes

641248

recombinant C-terminally poly-His-tagged N-terminally truncated mutant_187-338 from Escherichia coli by ion exchange chromatography and gel filtration

Corynebacterium ammoniagenes

Q59263

739570

recombinant enzyme

2 entries

recombinant His-tagged enzyme RFK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by a protease, followed by ammonium sulfate fractionation, hydrophobic interaction chromatography, and dialysis. HsRFK is purified free of flavin and adenine ligands

Homo sapiens

Q969G6

759167

recombinant RFK module of enzyme FADS, DELTA(1-182)CaFADS, from Escherichia coli strain BL21(DE3)

Corynebacterium ammoniagenes

Q59263

737357

recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and ion exchange chromatography, followed by dialysis

Corynebacterium ammoniagenes

Q59263

737771

recombinant, His-tagged enzyme by nickel affinity chromatography, removal of His-tag by TEV-protease treatment

Homo sapiens

Q969G6

657963