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2.7.1.26: riboflavin kinase

This is an abbreviated version!
For detailed information about riboflavin kinase, go to the full flat file.

Word Map on EC 2.7.1.26

Reaction

ATP
+
riboflavin
=
ADP
 +
FMN

Synonyms

AtFMN/FHy, ATP: riboflavin kinase, ATP:riboflavin kinase, bifunctional riboflavin kinase/FMN adenylyltransferase, CaFADS, FAD synthetase, FADS, FK, flavokinase, flavokinase/FAD synthetase, flavokinase/flavin adenine dinucleotide synthetase, FMN adenylyltransferase, FMNAT, HsRFK, kinase, riboflavin, More, RFK, RibC, ribF, riboflavin kinase, riboflavin kinase/FMN adenylyltransferase, riboflavine kinase, RibR

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.26 riboflavin kinase

Cloned

Cloned on EC 2.7.1.26 - riboflavin kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplification, cloning and expression of ribR gene in Escherichia coli
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichis coli BL-21—pLysS (DE3), C-terminal glutathione S-transferase fusionprotein
-
expression of ribC gene in Escherichia coli
gene RFK, recombinant expression of His-tagged enzyme from codon-optimzed gene in Escherichia coli strain BL21(DE3)
gene ribF, individual expression of the riboflavin kinase, RFK, module of enzyme FAD synthetase, FADS, i.e. DELTA(1-182)CaFADS, in Escherichia coli strain BL21(DE3)
gene ribF, recombinant expression of C-terminally poly-His-tagged N-terminally truncated mutant in Escherichia coli
gene ribF, recombinant expression of the isolated RF module
gene ribF, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
overproduced in Escherichia coli
the functional overexpression of the individual domains in Escherichia coli establishes that the riboflavin kinase and FMN hydrolase activities reside, respectively, in the C-terminal (AtFMN) and N-terminal (AtFHy) domains of AtFMN/FHy
-