2.7.1.23: NAD+ kinase
This is an abbreviated version!
For detailed information about NAD+ kinase, go to the full flat file.
Reaction
Synonyms
Afnk, AtNADK1, AtNADK2, AtNADK3, ATP: NAD(H) 2'-phosphotransferase, ATP:NAD 2'-phosphotransferase, ATP:NAD+ 2'-phosphotransferase, BsNADK, C5orf33, C5orf33 protein, diphosphopyridine nucleotide kinase, diphosphppyridine kinase, DPN kinase, EC 2.7.1.23, GmNADK1, GmNADK2, GmNADK3, GmNADK4, GmNADK5, HsNADK, kinase (phosphorylating), nicotinamide adenine dinucleotide, kinase, nicotinamide adenine dinucleotide (phosphorylating), MJ0917, NAD kinase, NAD kinase 1, NAD kinase 2, NAD kinase2, NAD(H) kinase, NadF, NADHK, NADK, NADK1, NADK2, NADK3, NADP phosphatase/NAD kinase, native plant calcium- and calmodulin-dependent NAD+-kinase, nicotinamide adenine dinucleotide kinase, OsNADK1, PH1074, Poly(P)/ATP NAD kinase
ECTree
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Turnover Number on EC 2.7.1.23 - NAD+ kinase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
2.3
ADP
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
7.8
polyphosphate(20)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
7.3
polyphosphate(25)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
3.4
polyphosphate(45), polyphosphate(65)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
-
1.8
polyphosphate(75)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
-
8.7
Tetraphosphate
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
3
Triphosphate
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
additional information
additional information
-
the molecular activity is 360 per min, the activity per active centre is less than 60 per min, pH 7.4, 30°C
-
0.6
ATP
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 6.0 and 30°C
2.02
ATP
with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C
2.54
ATP
Corynebacterium glutamicum subsp. lactofermentum
-
wild type enzyme, at pH 7.5 and 30°C
2.68
ATP
with NADH as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C
20.51
ATP
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 7.5 and 30°C
29.2
ATP
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
0.5
NAD+
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 6.0 and 30°C
3.8
NAD+
Corynebacterium glutamicum subsp. lactofermentum
-
wild type enzyme, at pH 7.5 and 30°C
7.8
NAD+
-
in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
15.42
NAD+
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 7.5 and 30°C
27.1
NAD+
-
in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
