2.7.1.23: NAD+ kinase
This is an abbreviated version!
For detailed information about NAD+ kinase, go to the full flat file.
Reaction
+
=
+
Synonyms
Afnk, AtNADK1, AtNADK2, AtNADK3, ATP: NAD(H) 2'-phosphotransferase, ATP:NAD 2'-phosphotransferase, ATP:NAD+ 2'-phosphotransferase, BsNADK, C5orf33, C5orf33 protein, diphosphopyridine nucleotide kinase, diphosphppyridine kinase, DPN kinase, EC 2.7.1.23, GmNADK1, GmNADK2, GmNADK3, GmNADK4, GmNADK5, HsNADK, kinase (phosphorylating), nicotinamide adenine dinucleotide, kinase, nicotinamide adenine dinucleotide (phosphorylating), MJ0917, NAD kinase, NAD kinase 1, NAD kinase 2, NAD kinase2, NAD(H) kinase, NadF, NADHK, NADK, NADK1, NADK2, NADK3, NADP phosphatase/NAD kinase, native plant calcium- and calmodulin-dependent NAD+-kinase, nicotinamide adenine dinucleotide kinase, OsNADK1, PH1074, Poly(P)/ATP NAD kinase , poly(P)/ATP-NAD kinase, poly(P)/ATPdependent NAD kinase, polyP/ATP-dependent NAD kinase, polyphosphate/ATP-NAD kinase, Pos5, Ppnk, PPNK_THEMA, sll1415, SlNADK1, SlNADK2, slr0040, slr0400, slr1415, SpNADK1, SpNADK2, styNadK, Utr, Utr1, Utr1p, YEF1, Yef1p, YfjB, YjbN, YtdI
ECTree
KCat KM Value
KCat KM Value on EC 2.7.1.23 - NAD+ kinase
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0.5
ADP
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
56.48
NADH
in 100 mM Tris-HCl (pH 7.5), at 37°C
1.3
polyphosphate(20)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
0.42
polyphosphate(25)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
0.24
polyphosphate(45)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
-
0.19
polyphosphate(65)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
-
0.58
polyphosphate(75)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
-
4.1
Tetraphosphate
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
1.2
Triphosphate
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
0.24
ATP
-
wild type enzyme, at pH 7.5 and 30°C
0.56
ATP
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 6.0 and 30°C
1.16
ATP
with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C
1.3
ATP
Corynebacterium glutamicum subsp. lactofermentum
-
wild type enzyme, at pH 7.5 and 30°C
5.5
ATP
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 7.5 and 30°C
6.72
ATP
with NADH as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C
59.6
ATP
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
0.36
NAD+
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 6.0 and 30°C
0.39
NAD+
-
wild type enzyme, at pH 7.5 and 30°C
0.94
NAD+
Corynebacterium glutamicum subsp. lactofermentum
-
wild type enzyme, at pH 7.5 and 30°C
1.2
NAD+
in 100 mM Tris-HCl (pH 7.5), at 37°C
7.05
NAD+
-
in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C
7.32
NAD+
Corynebacterium glutamicum subsp. lactofermentum
-
mutant enzyme P57S/P117S, at pH 7.5 and 30°C
40.5
NAD+
-
in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C