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2.7.1.2: glucokinase

This is an abbreviated version!
For detailed information about glucokinase, go to the full flat file.

Word Map on EC 2.7.1.2

Reaction

ATP
+
D-glucose
=
ADP
+
D-glucose 6-phosphate

Synonyms

ATP-dependent glucokinase, ATP-GLK, GK, GlcK, glk, Glk1, glucokinase (phosphorylating), glucokinase 1, kinase, gluco- (phosphorylating), KlGlk1, KLLA0C01155g, LmGlcK, NagC, NfGlck, SgGlkA, Sw0678, Sw2155, Sw3773, TcGlcK, TTE0090

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.2 glucokinase

Purification

Purification on EC 2.7.1.2 - glucokinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged recombinant TcGlcK is expressed in the Escherichia coli BL21 strain and purified to homogeneity by nickel affinity chromatography
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment and Ni2+ chelating affinity chromatography
-
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain RB791 by nickel affinity chromatography
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and gel filtration to homogeneity
-
using Ni-NTA chromatography