2.7.1.197: protein-Npi-phosphohistidine-D-mannitol phosphotransferase

This is an abbreviated version!
For detailed information about protein-Npi-phosphohistidine-D-mannitol phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.197

2.7.1.197

hpr

iicmtl

robillard

membrane-embedded

transphosphorylation

phospho-hpr

histidine-containing

carnosus

jacobson

pep-dependent

saier

activity-linked

phosphoenolpyruvate:sugar

Reaction

Synonyms

D-mannitol PTS permease, D-rnannitol-specific enzyme I1, EC 2.7.1.69, EII mtl, EIIMtl, enzyme IImtl, EnzymeIImtl, IIAmtl, IIBmtl, IImtl, ma nnitol-specific enzyme II, mannitol enzyme I1, mannitol permease, mannitol specific enzyme II, mannitol transporter, mannitol-specific carrier, mannitol-specific EII, mannitol-specific enzyme I1, mannitol-specific enzyme II, mannitol-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system, mannitol-specific enzyme IImtl, mannitolspecific enzyme II of the phosphotransferase system, mtlA, mtlI, phosphoenolpyruvate-dependent mannitol-specific transport protein, phosphotransferase IIAmannitol

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.197 protein-Npi-phosphohistidine-D-mannitol phosphotransferase

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Results	in table
5	Activating Compound
6529	AA Sequence
4	Cloned(Commentary)
1	Crystallization (Commentary)
26	Inhibitors
11	KM Value [mM]
14	Localization
1	Metals/Ions
14	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
29	Organism
2	Pathway
4	pH Optimum
3	Posttranslational Modification
17	Protein Variants
9	Purification (Commentary)
1	Reaction
25	Reference
2	Specific Activity [micromol/min/mg]
33	Substrates and Products (Substrate)
10	Subunits
61	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Engineering

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Engineering on EC 2.7.1.197 - protein-Npi-phosphohistidine-D-mannitol phosphotransferase

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C384S

Caldanaerobacter subterraneus subsp. tengcongensis

active site mutant of enzyme domain B

762334

C395S

Caldanaerobacter subterraneus subsp. tengcongensis

active site mutant of enzyme domain B

C384D

inactive

C384H

E218A

the mutant has a high Km for mannitol but can carry out facilitated diffusion of mannitol at a rate higher than the wild type protein in its unphosphorylated form

736381

E257A

Escherichia coli

P00550

the mutant does not detectably bind mannitol up to a concentration of at least 1 mM

736381

G253E

Escherichia coli

P00550

the mutant cannot transport but still can phosphorylate mannitol

736381

H156A

Escherichia coli

P00550

the mutant shows wild type activity

H195A

H195N

the mutant shows 30% of wild type activity

H195R

H554A

H554D

inactive