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2.7.1.19: phosphoribulokinase

This is an abbreviated version!
For detailed information about phosphoribulokinase, go to the full flat file.

Word Map on EC 2.7.1.19

Reaction

ATP
+
D-ribulose 5-phosphate
=
ADP
+
D-ribulose 1,5-bisphosphate

Synonyms

5-phosphoribulose kinase, AT1G32060, AtPRK, CrPRK, glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase, kinase, phosphoribulo- (phosphorylating), Mext_0980, phosphopentokinase, phosphoribulokinase, PKK, Prk, PrkA, PRuK, ribulose 5-phosphate kinase, ribulose phosphate kinase, ribulose-5-phosphate kinase, SePRK, synpcc7942_0977

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.19 phosphoribulokinase

Storage Stability

Storage Stability on EC 2.7.1.19 - phosphoribulokinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, stable
-
-20°C, 50 mM Tris-HCl buffer, pH 8.0, 1 mM 2-mercaptoethanol
-
-20°C, no loss of activity for 4 weeks
-
-80°C, 10 mM bicine-KOH buffer, pH 7.8, 100 mM KCl, 5 mM dithiothreitol, several months
-
-80°C, 100 mM Tris buffer, pH 8.0, 0.5 mM dithiothreitol, 10% v/v glycerol
-
-80°C, N2-atmosphere, 50 mM bicine-KOH, 10 mM potassium phosphate, 10 mM dithiothreitol, 1 mM EDTA, 10% v/v glycerol, 2 months
-
0-4°C, 100 mM imidazole-HCl buffer, pH 7.0, 10 mM MgCl2, 1 mM EDTA, 1 mM dithioerythritol, 3 months
-
0-4°C, no significant loss of activity after 1 month
-
0-4°C, pH 6.8, dithiothreitol, EDTA, 4 weeks
-
4°C, 0.02 M triethanolamine-HCl buffer, pH 7.6, 0.5 mM EDTA, 0.5 mM dithiothreitol, 4 months
-