2.7.1.177: L-threonine kinase
This is an abbreviated version!
For detailed information about L-threonine kinase, go to the full flat file.
Word Map on EC 2.7.1.177
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2.7.1.177
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enterica
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metal-binding
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adocbl
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cobamide
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mazei
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cobyric
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o-phosphate
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methanosarcina
- 2.7.1.177
- enterica
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metal-binding
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adocbl
- cobamide
- mazei
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cobyric
- o-phosphate
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methanosarcina
Reaction
Synonyms
bluE, L-Thr kinase, L-Thr kinase/L-Thr-P decarboxylase, L-Thr kinase/L-Thr-phosphate decarboxylase, MmCobD, More, PduX, RsBluE, RSP_0788
ECTree
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General Information
General Information on EC 2.7.1.177 - L-threonine kinase
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evolution
malfunction
metabolism
physiological function
additional information
Rhodobacterales lack pduX, instead they have the non-orthologous bluE gene that encodes an enzyme that, unlike PduX, is specific for L-Thr and cannot use L-Ser. Phylogenetic analysis. The RsBluE enzyme is found to be restricted to a subclass of Rhodobacterales with genetic attributes that indicate they have a strong preference for AdoCbl. That is to say, these Rhodobacter species prefer and synthesize only a cobamide (Cba) with DMB as the lower ligand base and AP-P as the nucleotide linker, Coalpha-(alpha-5,6-dimethylbenzimidazolyl-Cobeta-adenosylcobamide) (a.k.a. adenosylcobalamin, AdoCbl, coenzyme B12, CoB12). Cells with incomplete AdoCbl production have a pigmentation phenotype
evolution
the CobD protein from Methanosarcina mazei differs from other CobD homologues by the presence of a 111-amino acid cysteine-rich extended C-terminus (MmCobD386-497) annotated as a putative metal-binding domain or zinc finger protein, but it actually is a ferroprotein. This C-terminal domain is sometimes encoded as an independent protein and other times fused to other Cba biosynthetic proteins (e.g. CbiZ, CbiA, CbiH, or BtuC)
evolution
Cereibacter sphaeroides ATCC 17023
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Rhodobacterales lack pduX, instead they have the non-orthologous bluE gene that encodes an enzyme that, unlike PduX, is specific for L-Thr and cannot use L-Ser. Phylogenetic analysis. The RsBluE enzyme is found to be restricted to a subclass of Rhodobacterales with genetic attributes that indicate they have a strong preference for AdoCbl. That is to say, these Rhodobacter species prefer and synthesize only a cobamide (Cba) with DMB as the lower ligand base and AP-P as the nucleotide linker, Coalpha-(alpha-5,6-dimethylbenzimidazolyl-Cobeta-adenosylcobamide) (a.k.a. adenosylcobalamin, AdoCbl, coenzyme B12, CoB12). Cells with incomplete AdoCbl production have a pigmentation phenotype
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evolution
Cereibacter sphaeroides NCIB 8253
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Rhodobacterales lack pduX, instead they have the non-orthologous bluE gene that encodes an enzyme that, unlike PduX, is specific for L-Thr and cannot use L-Ser. Phylogenetic analysis. The RsBluE enzyme is found to be restricted to a subclass of Rhodobacterales with genetic attributes that indicate they have a strong preference for AdoCbl. That is to say, these Rhodobacter species prefer and synthesize only a cobamide (Cba) with DMB as the lower ligand base and AP-P as the nucleotide linker, Coalpha-(alpha-5,6-dimethylbenzimidazolyl-Cobeta-adenosylcobamide) (a.k.a. adenosylcobalamin, AdoCbl, coenzyme B12, CoB12). Cells with incomplete AdoCbl production have a pigmentation phenotype
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evolution
Cereibacter sphaeroides DSM 158
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Rhodobacterales lack pduX, instead they have the non-orthologous bluE gene that encodes an enzyme that, unlike PduX, is specific for L-Thr and cannot use L-Ser. Phylogenetic analysis. The RsBluE enzyme is found to be restricted to a subclass of Rhodobacterales with genetic attributes that indicate they have a strong preference for AdoCbl. That is to say, these Rhodobacter species prefer and synthesize only a cobamide (Cba) with DMB as the lower ligand base and AP-P as the nucleotide linker, Coalpha-(alpha-5,6-dimethylbenzimidazolyl-Cobeta-adenosylcobamide) (a.k.a. adenosylcobalamin, AdoCbl, coenzyme B12, CoB12). Cells with incomplete AdoCbl production have a pigmentation phenotype
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inactivation of gene bluE in Rhodobacter sphaeroides causes AdoCbl-dependent growth phenotypes, DELTAbluE strains fail to grow compared to bluE+ controls. A blush phenotype for the DELTAbluE strain is observed and the amount of light-harvesting complex 1 (LH1 B875) reaction center pigments of this strain are reduced relative to those in cultures of the DELTAbluE strain growing in the presence of AdoCbl after measurements are normalized for the cell density of each culture. The blush phenotype is also corrected by the addition of CNCbl to the medium
malfunction
there is a 2600fold decrease in catalytic efficiency (kcat/Km) when the C-terminus is removed, or a 1200fold decrease when the enzyme is purified normoxically
malfunction
Cereibacter sphaeroides ATCC 17023
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inactivation of gene bluE in Rhodobacter sphaeroides causes AdoCbl-dependent growth phenotypes, DELTAbluE strains fail to grow compared to bluE+ controls. A blush phenotype for the DELTAbluE strain is observed and the amount of light-harvesting complex 1 (LH1 B875) reaction center pigments of this strain are reduced relative to those in cultures of the DELTAbluE strain growing in the presence of AdoCbl after measurements are normalized for the cell density of each culture. The blush phenotype is also corrected by the addition of CNCbl to the medium
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malfunction
Cereibacter sphaeroides NCIB 8253
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inactivation of gene bluE in Rhodobacter sphaeroides causes AdoCbl-dependent growth phenotypes, DELTAbluE strains fail to grow compared to bluE+ controls. A blush phenotype for the DELTAbluE strain is observed and the amount of light-harvesting complex 1 (LH1 B875) reaction center pigments of this strain are reduced relative to those in cultures of the DELTAbluE strain growing in the presence of AdoCbl after measurements are normalized for the cell density of each culture. The blush phenotype is also corrected by the addition of CNCbl to the medium
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malfunction
Cereibacter sphaeroides DSM 158
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inactivation of gene bluE in Rhodobacter sphaeroides causes AdoCbl-dependent growth phenotypes, DELTAbluE strains fail to grow compared to bluE+ controls. A blush phenotype for the DELTAbluE strain is observed and the amount of light-harvesting complex 1 (LH1 B875) reaction center pigments of this strain are reduced relative to those in cultures of the DELTAbluE strain growing in the presence of AdoCbl after measurements are normalized for the cell density of each culture. The blush phenotype is also corrected by the addition of CNCbl to the medium
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involved in the biosynthesis of adenosylcobalamin
metabolism
enzyme BluE is the L-Thr kinase involved in AdoCbl biosynthesis in Rhodobacterales
metabolism
Cereibacter sphaeroides ATCC 17023
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enzyme BluE is the L-Thr kinase involved in AdoCbl biosynthesis in Rhodobacterales
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metabolism
Cereibacter sphaeroides NCIB 8253
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enzyme BluE is the L-Thr kinase involved in AdoCbl biosynthesis in Rhodobacterales
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metabolism
Cereibacter sphaeroides DSM 158
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enzyme BluE is the L-Thr kinase involved in AdoCbl biosynthesis in Rhodobacterales
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MmCobD is a bifunctional enzyme with L-threonine (L-Thr) kinase (PduX, EC 2.7.1.177) and pyridoxal 5'-phosphate (PLP)-dependent L-threonine phosphate (L-Thr-P) decarboxylase activities needed to synthesize the (R)-1-amino-propan-2-ol O-phosphate (a.k.a. (R)-1-amino-2-propanol-O-2-phosphate, AP-P) moiety of cobalamine (Cbl)
physiological function
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MmCobD is a bifunctional enzyme with L-threonine (L-Thr) kinase (PduX, EC 2.7.1.177) and pyridoxal 5'-phosphate (PLP)-dependent L-threonine phosphate (L-Thr-P) decarboxylase activities needed to synthesize the (R)-1-amino-propan-2-ol O-phosphate (a.k.a. (R)-1-amino-2-propanol-O-2-phosphate, AP-P) moiety of cobalamine (Cbl)
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