2.7.1.163: hygromycin B 4-O-kinase
This is an abbreviated version!
For detailed information about hygromycin B 4-O-kinase, go to the full flat file.
Word Map on EC 2.7.1.163
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2.7.1.163
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agrobacterium
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molecular biology
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agrobacterium-mediated
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explants
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biotechnology
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tumefaciens
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tumefaciens-mediated
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hygromycin-resistant
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acetosyringone
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plantlets
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cocultivation
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microalgal
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embryogenic
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pcambia
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majus
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nptii
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protocorms
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calluses
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orchid
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reintegration
- 2.7.1.163
- agrobacterium
- molecular biology
-
agrobacterium-mediated
-
explants
- biotechnology
- tumefaciens
-
tumefaciens-mediated
-
hygromycin-resistant
- acetosyringone
-
plantlets
-
cocultivation
-
microalgal
-
embryogenic
-
pcambia
- majus
- nptii
-
protocorms
-
calluses
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orchid
-
reintegration
Reaction
Synonyms
Hm phosphotransferase, HPH, Hph5, HPT, hptII, hygromycin B phosphotransferase, hygromycin B4-O-phosphohygromycin, hygromycin phosphotransferase, hygromycin-B kinase, hygromycin-phosphotransferase, HyR
ECTree
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Organism
Organism on EC 2.7.1.163 - hygromycin B 4-O-kinase
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Agrobacterium tumefaciens EHA101 (pRIT1) is used for transformation and the vector pRIT1 has been described previously. T-DNA of pRIT 1 carries the highly active rice Actin 1 promoter with its intron 1 fused with an hpt gene modified at its 3' coding region and the cauliflower virus.
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Agrobacterium tumefaciens strain C58C1 Rif-R harbouring a cointegrate plasmid pGV2260::pSSJ1 and a binary plasmid pCam-chi11 is used for co-transformation in rice. The T-DNA of the cointegrate vector pGV2260::pSSJ1 carries hph gene from Escherichia coli.
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binary plasmid pIG121Hm containing hpt gene is transformed into Marchantia polymorpha via Agrobacterium tumefaciens (strain GV2260)
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Binary vector pCAMBIA-1305.2 contains hphII gene desenced from Escherichia coli, transformed via Agrobacterium tumefaciens
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Binary vector plasmid pIG121Hm containing hpt gene descending from Escherichia coli.
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hph gene detected in two unapproved transgenic rice lines contaminating vermicelli products
UniProt
Protein sequence is used (vector pCAMBIA1380) for a selectable marker for soybean transformation selection.
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sequence is used in a vector by using the Agrobacterium-mediated transformation method
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strain BL21 (mutant enzyme Hph5 contains 5 amino-acid substitutions with increased thermostability of approximately -257°C in vivo compared with the wild-type protein), and methionine-auxotroph strain B834 (DE3)
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The vector pBHt2 contains a T-DNA harboring hygromycin B resistance (hygromycin phosphotransferase, hph) in the backbone of pCAMBIA1300 descended from Escherichia coli, transformed in Colletrichum acutatum and Colletrichum falcatum via Agrobacterium tumefaciens
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used strains are K-12 derivatives, isolating of a plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B
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