2.7.1.163: hygromycin B 4-O-kinase

This is an abbreviated version!
For detailed information about hygromycin B 4-O-kinase, go to the full flat file.

Word Map on EC 2.7.1.163

2.7.1.163

agrobacterium

molecular biology

agrobacterium-mediated

explants

biotechnology

tumefaciens

tumefaciens-mediated

hygromycin-resistant

acetosyringone

plantlets

cocultivation

microalgal

embryogenic

pcambia

majus

nptii

protocorms

calluses

orchid

reintegration

Reaction

Synonyms

Hm phosphotransferase, HPH, Hph5, HPT, hptII, hygromycin B phosphotransferase, hygromycin B4-O-phosphohygromycin, hygromycin phosphotransferase, hygromycin-B kinase, hygromycin-phosphotransferase, HyR

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.163 hygromycin B 4-O-kinase

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Results	in table
25	AA Sequence
20	Application
1	CAS Registry Number
19	Cloned(Commentary)
5	Cofactor
2	Crystallization (Commentary)
1	General Stability
3	KM Value [mM]
6	Metals/Ions
5	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
20	Organism
8	PDB ID
3	pH Optimum
1	pH Stability
21	Protein Variants
5	Purification (Commentary)
2	Reaction
3	Reaction Type
19	Reference
1	Renatured (Commentary)
12	Source Tissue
15	Specific Activity [micromol/min/mg]
13	Substrates and Products (Substrate)
1	Subunits
34	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
3	Temperature Stability [°C]
4	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 2.7.1.163 - hygromycin B 4-O-kinase

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ATP + hygromycin B

ADP + 4-O-phosphohygromycin B

Escherichia coli

phosphorylated product procures hygromcin B resistance

684479

ATP + hygromycin B

ADP + 7''-O-phosphohygromycin B

Escherichia coli

661543, 663314

hygromycin B + ATP

4-O-phosphohygromycin B + ADP

2 entries

hygromycin B + ATP

7''-O-phosphohygromycin B + ADP

Escherichia coli

resulting in a loss of cell-kill antibiotic activity of hygromycin B

671134

additional information

Escherichia coli

no phosphorylation of neomycin, kanamycin A and B, streptomycin, dideoxykanamycin B, tobramycin, gentamicin, G418, sisomicin, netilmicin, amikacin, apramycin, ribostamycin, butirosin, lividomycin, and paromomycin by this enzyme

684479