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2.7.1.158: inositol-pentakisphosphate 2-kinase

This is an abbreviated version!
For detailed information about inositol-pentakisphosphate 2-kinase, go to the full flat file.

Word Map on EC 2.7.1.158

Reaction

ATP
+
1D-myo-inositol 1,3,4,5,6-pentakisphosphate
=
ADP
+
1D-myo-inositol hexakisphosphate

Synonyms

ASP1, At5g42810, AtIP5 2-K, AtIPK1, CnIpk1, Glyma04g03240, Glyma06g03310, GmIpk1, GmlPk1, Gsl1p, inositol 1,3,4,5,6-pentakis phosphate 2-kinase, inositol 1,3,4,5,6-pentakisphosphate 2-kinase, inositol hexakisphosphate kinase 1, inositol kinase, inositol pentakisphosphate 2-kinase, inositol polyphosphate kinase, inositol polyphosphate kinase-1, inositol polyphosphate kinase/phosphotransferase, inositol-1,3,4,5,6-pentakisphosphate 2-kinase, ins(1,3,4,5,6)P5 2-kinase, Ins5 2-kinase, InsP5 2-kinase, InsP6-kinase, IP5 2-K, IP5 2-kinase, IP5K, IP6K, IP6K1, Ipk1, Ipk1p, IPP2K, mIP5 2-K, Plc1p, rIPK1, scIpk1, spIpk1-C, StITPK1, StITPKalpha, ZmIPK1, ZmIPK1A, ZmIPK1B

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.158 inositol-pentakisphosphate 2-kinase

Engineering

Engineering on EC 2.7.1.158 - inositol-pentakisphosphate 2-kinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D368A
E82C/S142C
-
the mutant exhibits a dithiothreitol-sensitive 5fold increase in kcat for 3,4,5,6-inositol tetrakisphosphate compared to the wild type enzyme
H196A
the mutant shows reduced activity compared to the wild type enzyme
K168A
K200A
the mutant shows reduced activity compared to the wild type enzyme
N238A
the mutant shows reduced activity compared to the wild type enzyme
N239A
the mutant shows reduced activity compared to the wild type enzyme
R192A
the mutant shows reduced activity compared to the wild type enzyme
R415A
the mutant shows reduced activity compared to the wild type enzyme
R45A
the mutant shows reduced activity compared to the wild type enzyme
Y419A
the mutant shows reduced activity compared to the wild type enzyme
Q64A/E66A/E67A
C162Y
-
inactive
D437A
site-directed mutagenesis, the mutant enzyme shows highly reduced activity
D439A
site-directed mutagenesis, nearly inactive mutant enzyme
K138A
site-directed mutagenesis, the mutant enzyme shows highly reduced activity
D333A
-
kinase dead mutant of Asp1
H397A
-
phosphatase dead mutant of Asp1
additional information